Effects of phenobarbital on aniline metabolism in primary liver cell culture of rats with ethionine-induced liver disorder
| Autor: | Shinji Nitoh, Michishige Noguchi, Yoshiaki Kawai, Masanari Mabuchi |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
medicine.medical_specialty
Aminophenols General Biochemistry Genetics and Molecular Biology Liver disorder Rats Sprague-Dawley chemistry.chemical_compound Internal medicine medicine Animals Ethionine Phenylhydroxylamine Cells Cultured Chromatography High Pressure Liquid Acetaminophen chemistry.chemical_classification Aniline Compounds General Veterinary Chemistry Liver cell Liver Diseases General Medicine Metabolism Culture Media Rats Kinetics Enzyme Endocrinology Liver Cell culture Phenobarbital Animal Science and Zoology Female Chemical and Drug Induced Liver Injury medicine.drug |
| Zdroj: | Experimental animals. 45(2) |
| ISSN: | 1341-1357 |
| Popis: | In experiment 1, the amount of aniline (AN) metabolites in the primary cell culture medium of the liver cells obtained from ethionine (ET)-treated rats was compared with that of the control (normal) rats. Although the metabolites detected in both groups were p-aminophenol (p-AP), N-acetyl-p-AP (AAP), acetoanilide (AAN), AAP-glucuronide (AAPG), phenylhydroxylamine sulfate (PHAS) and p-AP-glucuronide (p-APG), the amount of AAP was lower and that of p-APG was markedly higher in the ET-treated rats than in the control rats. In experiment 2, phenobarbital (PB) was orally administered to the ET-treated and control rats at a dose of 100 mg/kg. The time course changes in AN metabolites in the primary cell culture medium of liver cells obtained at 2 or 48 hr after PB treatment were compared with those without PB treatment. In the ET-treated rats, the amount of PHAS was slightly higher at 2 hr after PB treatment, and that of AAP was lower and that of p-APG was higher at 48 hr after PB treatment as compared with those without PB treatment. In the control rats, the amounts of AAP, AAN, p-AP and p-APG at 2 hr after PB treatment remained lower than those without PB treatment, and that of AAP was markedly lower and that of p-APG was higher at 48 hr after PB treatment as compared with those without PB treatment. These findings indicated greater detoxication in the primary liver cell culture in the ET-treated rats than in the control rats. Furthermore, detoxication was greater in the primary cell culture of liver cell obtained from the ET-treated rats after PB treatment than from those without PB treatment, because the production of acetylates (AAP) decreased and p-APG increased (induction of conjugated enzyme) in the PB treatment group. |
| Databáze: | OpenAIRE |
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