Identification of a Five-Gene Prognostic Signature Related to B Cells Infiltration in Pancreatic Adenocarcinoma
| Autor: | Haixing Jiang, Shao-mei Tang, Xiaoliang Huang, Shanyu Qin |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Oncology
B cells medicine.medical_specialty Stromal cell Receiver operating characteristic WGCNA Proportional hazards model business.industry medicine.medical_treatment Cancer International Journal of General Medicine General Medicine Immunotherapy medicine.disease Immune system Internal medicine pancreatic adenocarcinoma medicine Adenocarcinoma prognostic signature ssGSEA business Gene Original Research |
| Zdroj: | International Journal of General Medicine |
| ISSN: | 1178-7074 |
| DOI: | 10.2147/ijgm.s324432 |
| Popis: | Shaomei Tang,1 Xiaoliang Huang,2 Haixing Jiang,1 Shanyu Qin1 1Department of Gastroenterology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Peopleâs Republic of China; 2Department of Gastrointestinal Surgery, Guangxi Medical University Cancer Hospital, Nanning, Peopleâs Republic of ChinaCorrespondence: Shanyu QinDepartment of Gastroenterology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Peopleâs Republic of ChinaEmail qsy0511@163.comBackground: Pancreatic adenocarcinoma (PAAD) is an extremely malignant cancer. Immunotherapy is a promising avenue to increase the survival time of patients with PAAD.Methods: RNA sequencing and clinical data for PAAD were downloaded from the TCGA database. The ssGSEA method and weighted gene co-expression network analysis were used to calculate the relative abundance of tumor-infiltrating immune cells and identify the related modules. Least absolute shrinkage and selection operator (LASSO) and Cox regression analyses were used to construct a prognostic model. MCPcounter and EPIC were also used to assess immune cell components using gene expression profiles.Results: The B cells closely related module was identified, and five genes, including ARID5A, CLEC2B, MICAL1, MZB1, and RAPGEF1, were ultimately selected to establish a prognostic signature to calculate the risk scores of PAAD patients. KaplanâMeier curves showed worse survival in the high-risk patients (p < 0.05), and the area under the receiver operating characteristic (ROC) curves of risk score for 1-year and 3-year survival were 0.78 and 0.80, respectively, based on the training set. Similar results were verified using the validated and combined sets. Interestingly, the low-risk group presented significantly elevated immune and stromal scores, proportion of B cells, and associations between these five genes and B cells were identified using multiple methods including ssGSEA, MCPcounter, and EPIC.Conclusion: This is the first attempt to study a B cells-related prognostic signature, which is instrumental in the exploration of novel prognostic biomarkers in PAAD.Keywords: B cells, prognostic signature, WGCNA, ssGSEA, pancreatic adenocarcinoma |
| Databáze: | OpenAIRE |
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