Time-resolved X-ray diffraction studies on stretch-activated insect flight muscle
| Autor: | Katrina J. V. Poole, Roger S. Goody, K. Güth, Gert Rapp, Y. Maeda |
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| Rok vydání: | 1991 |
| Předmět: |
Mass distribution
Physiology Chemistry Muscles Synchrotron radiation Zonal and meridional Cell Biology Biochemistry Insect flight Molecular physics Coleoptera Protein filament Actin Cytoskeleton Crystallography X-Ray Diffraction X-ray crystallography Time course medicine Animals Stress Mechanical medicine.symptom Muscle Contraction Muscle contraction |
| Zdroj: | Journal of Muscle Research and Cell Motility. 12:208-215 |
| ISSN: | 1573-2657 0142-4319 |
| DOI: | 10.1007/bf01774040 |
| Popis: | The specific feature of stretch activation of the indirect flight muscle of the tropical waterbug Lethocerus was used to correlate mechanical and structural aspects of muscle contraction. The time courses of the changes in intensities of the strongest equatorial reflections, the (10) and (20) and of the first meridional reflection at 14.5 nm-1 were monitored using synchrotron radiation as a high intensity X-ray source. The ratio of the intensities of the equatorial reflections, (I20/I10), which reflects the mass distribution within the filament lattice array, increases by about 10% relative to the Ca(2+)-activated level when a rapid stretch is imposed, compared with a 200% change seen when fibres change from the relaxed to the rigor state, while the spacing of the lattice planes decreases by about 1%. The intensity of the first meridional reflection at 14.5 nm-1 decreases by about 35% during stretch activation with a slightly faster time course than the delayed tension increase. The results suggest that the average structure of cycling crossbridges is different from that present in the rigor state. |
| Databáze: | OpenAIRE |
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