Viral load and high prevalence of HR-HPV52 and 58 types in black women from rural communities
| Autor: | Maria Carmen Lopes Ferreira Silva, Angélica Espinosa Miranda, Luciana Bueno de Freitas, Liliana Cruz Spano, Lays Paula Bondi Volpini, Jerusa Araújo Dias |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Adult Rural Population medicine.medical_specialty HPV Adolescent Genotype Population Black People Uterine Cervical Neoplasms Infectious and parasitic diseases RC109-216 Real-Time Polymerase Chain Reaction 03 medical and health sciences Young Adult 0302 clinical medicine Medical microbiology Risk Factors Internal medicine medicine Prevalence Humans Viral load Cervical cancer screening education Papillomaviridae Aged Cervical cancer education.field_of_study business.industry Papillomavirus Infections HPV infection Middle Aged medicine.disease Rural communities 030104 developmental biology Infectious Diseases Parasitology 030220 oncology & carcinogenesis DNA Viral Female Restriction fragment length polymorphism business Brazil Polymorphism Restriction Fragment Length Research Article |
| Zdroj: | BMC Infectious Diseases, Vol 21, Iss 1, Pp 1-8 (2021) BMC Infectious Diseases |
| ISSN: | 1471-2334 |
| Popis: | BackgroundThe high-risk human papillomavirus (HR-HPV) infection is the main cause of cervical cancer development, and the most common types were included in the last approved nonavalent vaccine (9vHPV). Geographical, socioeconomic and ethnic barriers in developing countries challenge primary and secondary prevention measures of cervical cancer. We aimed to determine the prevalence of HPV infection and the viral load of HR-HPV 9vHPV-related types black women resident in rural semi-isolated communities.MethodsA descriptive study was conducted with 273 cervical samples of women from rural communities of Southeastern Brazil. Viral DNA was amplified by PCR, the genotype was identified by Reverse Line Blot (RLB) and Restriction Fragment Length Polymorphism (RFLP), and real-time PCR was applied to determine the viral load.ResultsHPV frequency was 11.4% (31/273), associated with the presence of cytological abnormalities (32.3%;p 58, 59. Multiple infections occurred with up to nine different genotypes. The viral load of HR-HPV 9vHPV-related types was higher in lesions than in normal cytology cases (p = 0.04); “high” and “very high” viral load occurred in HSIL and LSIL, respectively (p = 0.04).ConclusionsWe highlight that despite the low HPV frequency in the black rural women population, the frequency of HR-HPV was high, particularly by the HR-HPV52 and 58 types. Moreover, the HR-HPV viral load increased according to the progression from normal to lesion, being a potential biomarker to identify those women at higher risk of developing cervical lesions in this population. |
| Databáze: | OpenAIRE |
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