Zfx Controls the Self-Renewal of Embryonic and Hematopoietic Stem Cells
| Autor: | Teresita L. Arenzana, Jose M. Galan-Caridad, Leonid A. Mirny, Z. Esther Hou, Sivan Harel, Fiona Doetsch, Boris Reizis |
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| Rok vydání: | 2007 |
| Předmět: |
Male
Cellular differentiation Kruppel-Like Transcription Factors Gene Expression Apoptosis Biology General Biochemistry Genetics and Molecular Biology Mice Downregulation and upregulation Proto-Oncogene Proteins Animals Progenitor cell Transcription factor Embryonic Stem Cells reproductive and urinary physiology Cell Proliferation Biochemistry Genetics and Molecular Biology(all) urogenital system Gene targeting Cell Differentiation Hematopoietic Stem Cells Embryonic stem cell Molecular biology Cell biology Haematopoiesis Gene Targeting embryonic structures Female biological phenomena cell phenomena and immunity Stem cell T-Box Domain Proteins |
| Zdroj: | Cell. 129:345-357 |
| ISSN: | 0092-8674 |
| DOI: | 10.1016/j.cell.2007.03.014 |
| Popis: | SummaryStem cells (SC) exhibit a unique capacity for self-renewal in an undifferentiated state. It is unclear whether the self-renewal of pluripotent embryonic SC (ESC) and of tissue-specific adult SC such as hematopoietic SC (HSC) is controlled by common mechanisms. The deletion of transcription factor Zfx impaired the self-renewal but not the differentiation capacity of murine ESC; conversely, Zfx overexpression facilitated ESC self-renewal by opposing differentiation. Furthermore, Zfx deletion abolished the maintenance of adult HSC but did not affect erythromyeloid progenitors or fetal HSC. Zfx-deficient ESC and HSC showed increased apoptosis and SC-specific upregulation of stress-inducible genes. Zfx directly activated common target genes in ESC and HSC, as well as ESC-specific target genes including ESC self-renewal regulators Tbx3 and Tcl1. These studies identify Zfx as a shared transcriptional regulator of ESC and HSC, suggesting a common genetic basis of self-renewal in embryonic and adult SC. |
| Databáze: | OpenAIRE |
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