Detection of hypothetical proteins in human fetal perireticular nucleus
| Autor: | Gert Lubec, Norbert Ulfig, Felix Hepner, and Arnold Pollak, Jae-Kyung Myung |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Male
Proteomics Hypothetical protein Apoptosis Gestational Age Computational biology Biology Biochemistry Mass Spectrometry chemistry.chemical_compound Pregnancy medicine Humans Electrophoresis Gel Two-Dimensional Databases Protein Fetal Death Gel electrophoresis Cell Cycle Brain Computational Biology Gene Expression Regulation Developmental Proteins General Chemistry Human brain DNA Cell cycle medicine.anatomical_structure chemistry Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Proteome Female Nucleus |
| Zdroj: | Journal of proteome research. 4(6) |
| ISSN: | 1535-3893 |
| Popis: | There is a legion of hypothetical proteins (HP) in prokaryotic and eukaryotic proteomes and the aim of this study was to describe HP in the perireticular nucleus (PN), a key structure in human brain development. Tissue from four PNs was homogenized and extracted proteins were run on two-dimensional gel electrophoresis followed by in-gel digestion and mass spectrometrical identification of proteins. Several databases were used for obtaining bioinformatic information and searching for functional and structural domains. Five spots represented HP: KIAA0423 protein (Q9Y4F4), hypothetical protein KIAA0153 (Q14166), hypothetical protein DKFZp564A2416 (Q9NTW4), hypothetical protein DKFZp564H1122 (Q9H0W9), and hypothetical protein DKFZp564D1378 (Q9H0R4). These structures were predicted to serve in cell cycle, DNA-condensation, neurogenesis, or apoptosis. The existence of formerly HP proteins in the PN of human fetal brain is shown, thus extending knowledge of the brain proteome and proposing the method used as a suitable analytical tool for searching HP. |
| Databáze: | OpenAIRE |
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