LEFKOTHEA Regulates Nuclear and Chloroplast mRNA Splicing in Plants
| Autor: | George Panayotou, Gerasimos Daras, Polydefkis Hatzopoulos, Stamatis Rigas, Dikran Tsitsekian, Anastasios Alatzas, Anna-Michaella Athanasiadou, Vassiliki Papadaki, Georgios Banilas, Martina Samiotaki, Dimitris Chatzopoulos, Vassiliki Kostourou, Dimitris Templalexis |
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| Rok vydání: | 2018 |
| Předmět: |
Spliceosome
Chloroplasts RNA Splicing Meristem Arabidopsis Biology Genes Plant Models Biological General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine Gene Expression Regulation Plant Gene expression RNA Precursors RNA Messenger Molecular Biology Post-transcriptional regulation 030304 developmental biology Cell Nucleus 0303 health sciences Arabidopsis Proteins Intron food and beverages RNA RNA-Binding Proteins Cell Biology Group II intron Introns Cell biology Chloroplast Phenotype RNA splicing Mutation Seeds Spliceosomes 030217 neurology & neurosurgery Developmental Biology Protein Binding |
| Zdroj: | Developmental cell. 50(6) |
| ISSN: | 1878-1551 |
| Popis: | Summary Eukaryotic organisms accomplish the removal of introns to produce mature mRNAs through splicing. Nuclear and organelle splicing mechanisms are distinctively executed by spliceosome and group II intron complex, respectively. Here, we show that LEFKOTHEA, a nuclear encoded RNA-binding protein, participates in chloroplast group II intron and nuclear pre-mRNA splicing. Transiently optimized LEFKOTHEA nuclear activity is fundamental for plant growth, whereas the loss of function abruptly arrests embryogenesis. Nucleocytoplasmic partitioning and chloroplast allocation are efficiently balanced via functional motifs in LEFKOTHEA polypeptide. In the context of nuclear-chloroplast coevolution, our results provide a strong paradigm of the convergence of RNA maturation mechanisms in the nucleus and chloroplasts to coordinately regulate gene expression and effectively control plant growth. |
| Databáze: | OpenAIRE |
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