TORC1 and TORC2 Coactivators Are Required for Tax Activation of the Human T-Cell Leukemia Virus Type 1 Long Terminal Repeats
| Autor: | Dong-Yan Jin, Kam-Leung Siu, King-Tung Chin, Elizabeth Yee Wai Choy, YT Siu, Kuan-Teh Jeang |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Gene Expression Regulation
Viral Transcription Genetic Torc viruses Immunology P300-CBP Transcription Factors Activating Transcription Factor 4 CREB Microbiology Transcription (biology) Virology Humans p300-CBP Transcription Factors Cyclic AMP Response Element-Binding Protein Transcription factor health care economics and organizations Genetics Human T-lymphotropic virus 1 biology Terminal Repeat Sequences Gene Products tax Phosphoproteins Coactivation Long terminal repeat Genome Replication and Regulation of Viral Gene Expression Insect Science Trans-Activators biology.protein Virus Activation HeLa Cells Protein Binding Transcription Factors |
| Zdroj: | Journal of Virology. 80:7052-7059 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.00103-06 |
| Popis: | Human T-cell leukemia virus type 1 (HTLV-1) Tax protein activates viral transcription from the long terminal repeats (LTR). Mechanisms through which Tax activates LTR have been established, but coactivators of this process remain to be identified and characterized. Here we show that all three members of the TORC family of transcriptional regulators are coactivators of Tax for LTR-driven expression. TORC coactivation requires CREB, but not ATF4 or other bZIP factors. Tax physically interacts with TORC1, TORC2, and TORC3 (TORC1/2/3), and the depletion of TORC1/2/3 inhibited Tax activity. TORC coactivation can be further enhanced by transcriptional coactivator p300. In addition, coactivators in the p300 family are required for full activity of Tax independently of TORC1/2/3. Thus, both TORC and p300 families of coactivators are essential for optimal activation of HTLV-1 transcription by Tax. |
| Databáze: | OpenAIRE |
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