Hydrated Human Corneal Stroma Revealed by Quantitative Dynamic Atomic Force Microscopy at Nanoscale
| Autor: | Shuai Zhang, Jesper Hjortdal, Flemming Besenbacher, Jacques Chevallier, Qiang Li, Karen Louise Thomsen, Mingdong Dong, Dan Xia |
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| Rok vydání: | 2014 |
| Předmět: |
Materials science
Fiber structure Corneal Stroma quantitative dynamic atomic force microscopy Analytical chemistry General Physics and Astronomy Nanoparticle Microscopy Atomic Force Stroma Elastic Modulus Cornea medicine Humans Nanotechnology General Materials Science Nanoscopic scale Mechanical Phenomena Mechanical property Atomic force microscopy General Engineering Stiffness Humidity Biomechanical Phenomena mechanical property medicine.anatomical_structure Biophysics corneal stroma Collagen medicine.symptom hydration |
| Zdroj: | Xia, D, Zhang, S, Hjortdal, J Ø, Li, Q, Thomsen, K, Chevallier, J, Besenbacher, F & Dong, MD 2014, ' Hydrated human corneal stroma revealed by quantitative dynamic atomic force microscopy at nanoscale ', ACS Nano, vol. 8, no. 7, pp. 6873-6882 . https://doi.org/10.1021/nn5015837 |
| ISSN: | 1936-086X 1936-0851 |
| Popis: | The structures and mechanical properties of human tissues are significantly influenced by water. The functionality of the human cornea can be linked to the hydrated collagen fibers. By applying quantitative dynamic atomic force microscopy to investigate morphological and mechanical property variations of corneal stroma under different hydration levels, we found that the collagen fibers in the stromal tissue show the specific periodicities and the stiffness of giga-Pa magnitude at 40% humidity. However, under increasing hydration, the collagen fibers clearly show nanoparticle structures along the fibers with the stiffness in mega-Pa magnitude. By increasing the hydration time, the stroma regains the fiber structure but with larger diameter. The age-dependency in stiffness was further investigated. The interplay of structures and nanomechanical mapping may be applied for the future diagnosis and assessment or even pathologic analysis. |
| Databáze: | OpenAIRE |
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