Chiral purity test of bevantolol by capillaryelectrophoresis and high performance liquid chromatography
| Autor: | Tran Quoc Trung, Joung Won Oh, Pham Hai Long, Kyeong Ho Kim |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Adrenergic beta-Antagonists
Analytical chemistry High-performance liquid chromatography Propanolamines chemistry.chemical_compound Capillary electrophoresis Drug Discovery medicine Enantiomeric excess Phosphoric acid Chromatography High Pressure Liquid Detection limit Chromatography Chemistry Organic Chemistry Electrophoresis Capillary Reproducibility of Results Stereoisomerism Reference Standards Chiral column chromatography Bevantolol Molecular Medicine Enantiomer Drug Contamination Algorithms medicine.drug |
| Zdroj: | Archives of Pharmacal Research. 29:808-813 |
| ISSN: | 1976-3786 0253-6269 |
| DOI: | 10.1007/bf02974083 |
| Popis: | Two methods for the chiral purity determination of bevantolol were developed, namely capillary electrophoresis (CE) using carboxymethyl-beta-cyclodextrin (CM-beta-CD) as a chiral selector and high-performance liquid chromatography (HPLC) using a chiral stationary phase. In the HPLC method, the separation of bevantolol enantiomers was performed on a Chiralpak AD-H column by isocratic elution with n-hexane-ethanol-diethylamine (10:90:0.1, v/v/v) as mobile phase. In the CE method, bevantolol enantiomers were separated on an uncoated fused silica capillary with 50 mM amonium phosphate dibasic adjusted to a pH 6.5 with phosphoric acid containing 15 mM CM-beta-CD as running buffer. Validation data such as linearity, recovery, detection limit, and precision of the two methods are presented. The detection limits of S-(-)-bevantolol were 0.1% and 0.05% for CE and HPLC method, respectively and R-(+)-bevantolol were 0.15% and 0.05% for CE and HPLC method, respectively. There was generally good agreement between the HPLC and CE results. |
| Databáze: | OpenAIRE |
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