Irradiation Induced Biochemical Changes in Human Mandibular Bone: A Raman Spectroscopic Study

Autor: Sridhar Reddy Padala, Dimple Saikia, Jopi J. W. Mikkonen, Emilia Uurasjärvi, Hannah Dekker, Engelbert A. J. M. Schulten, Nathalie Bravenboer, Arto Koistinen, Amrita Chauhan, Surya P. Singh, Arja M. Kullaa
Přispěvatelé: Oral and Maxillofacial Surgery / Oral Pathology, AMS - Tissue Function & Regeneration, Clinical chemistry, AMS - Ageing & Vitality, Amsterdam Gastroenterology Endocrinology Metabolism, Maxillofacial Surgery (AMC + VUmc), Maxillofacial Surgery (VUmc)
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Applied Spectroscopy, 76(10), 1165-1173. Society for Applied Spectroscopy
Applied Spectroscopy, 76(10), 1165-1173. SAGE PUBLICATIONS INC
Reddy Padala, S, Saikia, D, Mikkonen, J J W, Uurasjärvi, E, Dekker, H, Schulten, E A J M, Bravenboer, N, Koistinen, A, Chauhan, A, Singh, S P & Kullaa, A M 2022, ' Irradiation Induced Biochemical Changes in Human Mandibular Bone : A Raman Spectroscopic Study ', Applied Spectroscopy, vol. 76, no. 10, pp. 1165-1173 . https://doi.org/10.1177/00037028221109244
ISSN: 0003-7028
DOI: 10.1177/00037028221109244
Popis: Understanding the biochemical changes in irradiated human mandible after radiotherapy of cancer patients is critical for oral rehabilitation. The underlying mechanism for radiation-associated changes in the bone at the molecular level could lead to implant failure and osteoradionecrosis. The study aimed to assess the chemical composition and bone quality in irradiated human mandibular bone using Raman spectroscopy. A total of 33 bone biopsies from 16 control and 17 irradiated patients were included to quantify different biochemical parameters from the Raman spectra. The differences in bone mineral and matrix band intensities between control and irradiated groups were analyzed using unpaired Student’s t-test with statistical significance at p < 0.05. Findings suggest that the intensity of the phosphate band is significantly decreased and the carbonate band is significantly increased in the irradiated group. Further, the mineral crystallinity and carbonate to phosphate ratio are increased. The mineral to matrix ratio is decreased in the irradiated group. Principal component analysis (PCA) based on the local radiation dose and biopsy time interval of irradiated samples did not show any specific classification between irradiation sub-groups. Irradiation disrupted the interaction and bonding between the organic matrix and hydroxyapatite minerals affecting the bone biochemical properties. However, the normal clinical appearance of irradiated bone would have been accompanied by underlying biochemical and microscopical changes which might result in radiation-induced delayed complications. (Figure presented.).
Databáze: OpenAIRE
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