| Autor: |
Hoshino, Yoshiki, Sugihara, Takaaki, Ikeda, Suguru, Tarumoto, Ryohei, Matsuki, Yukako, Kanda, Tsutomu, Iyama, Takuji, Tomoaki Takata, Matono, Tomomitsu, Nagahara, Takakazu, Okano, Jun-Ichi, Ueki, Masaru, Koda, Masahiko, Osaki, Mitsuhiko, Okada, Futoshi, Isomoto, Hajime |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Web of Science |
| ISSN: |
1881-7742 |
| Popis: |
In cholestatic liver diseases, coagulopathy is induced by malabsorption of vitamin K. Supplementation of vitamin K has previously been shown to prevent coagulopathy. In this study, we tested the efficacy of a newly invented micellized vitamin K2 (m-vitK2) in treating coagulopathy, using a rat bile duct ligation (BDL) model. Experiment 1: m-vitK2 (0.3 mg/kg) or m-vitK2 (0.3 mg/kg) mixed with taurocholic acid (TA) (10 mg/body) was orally administrated every day for 7 d from the fourth day after BDL (n=6 for each). Experiment 2: To evaluate absorption, m-vitK2 (0.3 mg/kg) with or without TA (10 mg/body) was orally administered on the fourth day after BDL and compared with the untreated control BDL (n=2 for each). These data were compared with sham-operated (n=6) and untreated control BDL rats (n=6). The m-vitK2 recovered prothrombin time (PT) in Experiment 1 (control 42.7±5.7 s vs. m-vitK2 24.0±9.3 s, p0.05). Experiment 2 demonstrated that the mixture of m-vitK2 and TA enhanced absorption compared to m-vitK2 alone. Moreover, in Experiment 1, m-vitK2 mixed with TA completely recovered PT (control 42.7±5.7 s vs. m-vitK2+TA 14.9±1.2 s, p0.01). Micelle sizes decreased with the m-vitK2 and TA treatment (m-vitK2 86.3±5.6 nm vs. m-vitK2+TA 71.9±4.7 nm, p0.05). Orally administered, newly invented m-vitK2 recovered coagulopathy even under obstructive jaundice. TA decreased the mean micelle size and improved m-vitK2 absorption. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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