Multivalent Aptamer-RNA Conjugates for Simple and Efficient Delivery of Doxorubicin/siRNA into Multidrug-Resistant Cells
| Autor: | Heesun Jung, Soo Hyeon Lee, Sun Hwa Kim, Hyundong Yoo, Hyejung Mok, Hyosook Jeong, Yeonju Hwang |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Polymers and Plastics Anthracycline Aptamer Bioengineering 02 engineering and technology Biomaterials 03 medical and health sciences Drug Delivery Systems Materials Chemistry medicine Humans Doxorubicin Viability assay RNA Small Interfering Caspase 7 Chemistry Caspase 3 Transfection Aptamers Nucleotide 021001 nanoscience & nanotechnology Molecular biology 030104 developmental biology Spectrometry Fluorescence Cell culture Drug Resistance Neoplasm Cancer cell Nucleic acid Cancer research MCF-7 Cells RNA 0210 nano-technology Biotechnology medicine.drug |
| Zdroj: | Macromolecular bioscience. 17(4) |
| ISSN: | 1616-5195 |
| Popis: | Multivalent aptamer–siRNA conjugates containing multiple mucin-1 aptamers and BCL2-specific siRNA are synthesized, and doxorubicin, an anthracycline anticancer drug, is loaded into these conjugates through intercalation with nucleic acids. These doxorubicin-incorporated multivalent aptamer–siRNA conjugates are transfected to mucin-1 overexpressing MCF-7 breast cancer cells and their multidrug-resistant cell lines. Doxorubicin-incorporated multivalent aptamer–siRNA conjugates exert promising anticancer effects, such as activation of caspase-3/7 and decrease of cell viability, on multidrug-resistant cancer cells because of their high intracellular uptake efficiency. Thus, this delivery system is an efficient tool for combination oncotherapy with chemotherapeutics and nucleic acid drugs to overcome multidrug resistance. |
| Databáze: | OpenAIRE |
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