Sex and Genetic Background Influence Superoxide Dismutase (cSOD)-Related Phenotypic Variation in Drosophila melanogaster
| Autor: | Tony L. Parkes, Courtney E Lessel, Thomas J.S. Merritt, Joël Dickinson |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Genotype media_common.quotation_subject Biology QH426-470 Investigations medicine.disease_cause Superoxide dismutase genetic background 03 medical and health sciences 0302 clinical medicine Cytosol Malate Dehydrogenase medicine Genetics sex Animals Allele Molecular Biology Gene Genetics (clinical) Crosses Genetic media_common Genes Dominant Genetic diversity Sex Characteristics Superoxide Dismutase Longevity biology.organism_classification Phenotype Chromosomes Insect 030104 developmental biology Drosophila melanogaster Biological Variation Population biology.protein Female 030217 neurology & neurosurgery Oxidative stress Locomotion NADP |
| Zdroj: | G3: Genes|Genomes|Genetics G3: Genes, Genomes, Genetics, Vol 7, Iss 8, Pp 2651-2664 (2017) |
| ISSN: | 2160-1836 |
| Popis: | Mutations often have drastically different effects in different genetic backgrounds; understanding a gene’s biological function then requires an understanding of its interaction with genetic diversity. The antioxidant enzyme cytosolic copper/zinc superoxide dismutase (cSOD) catalyzes the dismutation of the superoxide radical, a molecule that can induce oxidative stress if its concentration exceeds cellular control. Accordingly, Drosophila melanogaster lacking functional cSOD exhibit a suite of phenotypes including decreased longevity, hypersensitivity to oxidative stress, impaired locomotion, and reduced NADP(H) enzyme activity in males. To date, cSOD-null phenotypes have primarily been characterized using males carrying one allele, cSodn108red, in a single genetic background. We used ANOVA, and the effect size partial eta squared, to partition the amount of variation attributable to cSOD activity, sex, and genetic background across a series of life history, locomotor, and biochemical phenotypes associated with the cSOD-null condition. Overall, the results demonstrate that the cSOD-null syndrome is largely consistent across sex and genetic background, but also significantly influenced by both. The sex-specific effects are particularly striking and our results support the idea that phenotypes cannot be considered to be fully defined if they are examined in limited genetic contexts. |
| Databáze: | OpenAIRE |
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