HIV Type 1 Subtypes among Blood Donors in the Mbeya Region of Southwest Tanzania

Autor:	Oliver Hoffmann, Eleuter Samky, Karl-Heinz Herbinger, Deborah L. Birx, Miguel A. Arroyo, Francine E. McCutchan, Leonard Maboko, Michael Hoelscher, Eric Sanders-Buell, Merlin R. Robb
Rok vydání:	2004
Předmět:	Genetic diversity biology Immunology Blood Donors HIV Infections Genome Viral Sequence Analysis DNA biology.organism_classification Tanzania Virology Peripheral blood mononuclear cell Virus Plasma Infectious Diseases DNA Viral Lentivirus HIV-1 Humans Viral disease Typing Sida Phylogeny
Zdroj:	AIDS Research and Human Retroviruses. 20:895-901
ISSN:	1931-8405 0889-2229
Popis:	HIV-1 is endemic in Tanzania where three different subtypes, A, C, and D, have been identified. Information on HIV-1 genetic diversity is crucial to define requirements for an effective vaccine, in regions where HIV-1 vaccine trials are planned. To define the subtype distribution of HIV-1 in the Mbeya region of southwest Tanzania, peripheral blood mononuclear cells (PBMC) and plasma were obtained from 36 discarded HIV seropositive blood units. Multiregion hybridization assay (MHA) was performed on both PBMC DNA and plasma RNA to determine the subtype distribution. Twenty virtually full-length HIV-1 sequences were amplified from the extracted DNA, sequenced, and phylogenetically analyzed. Subtype distribution determined by all three assays was comparable. More than 50% of the samples analyzed were subtype C, followed by a high proportion of subtype C-containing intersubtype recombinants. Based on this work, subtype C appears to be the prevalent subtype in southwest Tanzania, followed by a high proportion of intersubtype recombinants.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1d1e7dc443b6170685154af87e0b246a https://doi.org/10.1089/0889222041725235 Zobrazit plný text záznamu