Monoclonal Antibodies for Detection of Lymphocyte Markers: Comparison of Three Commercial Kits
| Autor: | Jorge F. Jimenez, Cynthia Cohen, Hazel V. Horn |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Pathology
medicine.medical_specialty medicine.drug_class T cell Palatine Tonsil Monoclonal antibody T-Lymphocytes Regulatory Leukocyte Count Humans Medicine False Positive Reactions Lymphocytes False Negative Reactions Frozen section procedure biology Histocytochemistry business.industry Becton dickinson Antibodies Monoclonal T-Lymphocytes Helper-Inducer General Medicine T lymphocyte Staining medicine.anatomical_structure biology.protein Reagent Kits Diagnostic Antibody business Immunostaining |
| Zdroj: | American Journal of Clinical Pathology. 85:438-444 |
| ISSN: | 1943-7722 0002-9173 |
| DOI: | 10.1093/ajcp/85.4.438 |
| Popis: | Three commercial monoclonal antibody kits (Histoset®, OrthoDiagnostics, Raritan, NJ; T Cell Panel®, Becton Dickinson, Mountain View, CA; Histotag® Lymphoma Set, Hybritech Inc., San Diego, CA) were compared, using touch preparations, frozen sections, and formalin-fixed paraffin-embedded sections of 14 tonsils. With the first two kits, sensitive results were obtained in touch preparations and frozen sections with strong, crisp, reproducible immunostain. Results were accurate and easily interpreted despite moderate background staining. Distribution and numbers of lymphocytes and subsets were appropriate. The Histotag® Lymphoma Set was sensitive for detection of T-cells in touch preparations and frozen sections. Hazy, moderate immunostain was obtained with B-cell and common leukocyte antibodies. Accuracy was questioned because of variable and false-negative results (75% in touch preparations) with all anti-sera, and interfollicular staining for B-lymphocytes. In formalin-fixed sections, positive results of low sensitivity were obtained only with the T Cell Panel®. The Histoset® and T Cell Panel® are recommended, particularly for use with frozen sections. |
| Databáze: | OpenAIRE |
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