Effects of shikonin on the development of ovarian follicles and female germline stem cells
| Autor: | Li-Bo Tang, Shu-Xin Ma, Yuehui Zheng, Zhi-Hang Chen, Jia Li, Guo Zong, Zi-Juan Tang, Min-Li Wei |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Medicine (General) Oogonial Stem Cells medicine.disease_cause Stem cell marker Biochemistry Germline Pre-Clinical Research Report reproduction Andrology Superoxide dismutase Mice 03 medical and health sciences 0302 clinical medicine R5-920 Ovarian Follicle ovarian follicular development Follicular phase medicine Animals oxidative stress Shikonin chemistry.chemical_classification Reactive oxygen species biology business.industry Biochemistry (medical) apoptosis Cell Biology General Medicine Antral follicle 030104 developmental biology chemistry Apoptosis 030220 oncology & carcinogenesis female germline stem cells biology.protein Female business Oxidative stress Naphthoquinones |
| Zdroj: | Journal of International Medical Research, Vol 49 (2021) The Journal of International Medical Research |
| ISSN: | 1473-2300 |
| Popis: | Objective To investigate the effects and potential mechanism of action of shikonin (SHK) on the development of ovarian follicles and female germline stem cells (FGSCs). Methods Female Kunming adult mice were administered SHK (0, 20 and 50 mg/kg) by oral gavage. Cultures of FGSCs were treated with SHK 32 μmol/l for 24 h. The ovarian index in mouse ovaries was calculated. Numbers of primordial, primary and atretic follicles were counted. Germline stem cell markers and apoptosis were examined. Levels of glutathione (GSH), superoxide dismutase (SOD) and reactive oxygen species (ROS) were measured. Results Both doses of SHK significantly decreased the ovarian index, the numbers of primordial follicles, primary follicles and antral follicles in mice. SHK significantly increased the numbers of atretic follicles and atretic corpora lutea. SHK promoted apoptosis in vivo and in vitro. SHK significantly decreased the levels of the germline stem cell markers. SHK significantly lowered GSH levels and the activity of SOD in the peripheral blood from mice, whereas SHK significantly elevated cellular ROS content in FGSCs. Conclusions These current results suggested that follicular development and FGSCs were suppressed by SHK through the induction of apoptosis and oxidative stress might be involved in this pathological process. |
| Databáze: | OpenAIRE |
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