A label-free reusable aptasensor for Alzheimer’s disease
Autor: | Gui-Yan Wu, Qilong Zhong, Yang-Bao Miao, Hui-Ling Wang, Ying Zhang, Hong-Xia Ren, Xiao-Wei Wen |
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Rok vydání: | 2020 |
Předmět: |
Aptamer
DNA Single-Stranded Nanoparticle Biosensing Techniques 02 engineering and technology 010402 general chemistry 01 natural sciences Oligomer Analytical Chemistry chemistry.chemical_compound Alzheimer Disease Lanthanum Limit of Detection Humans Magnetite Nanoparticles Protein Structure Quaternary Metal-Organic Frameworks Fluorescent Dyes Label free Amyloid beta-Peptides Immunomagnetic Separation Aptamers Nucleotide 021001 nanoscience & nanotechnology Serum samples Combinatorial chemistry 0104 chemical sciences Spectrometry Fluorescence chemistry Linear range 0210 nano-technology Luminescence Selectivity Antibodies Immobilized |
Zdroj: | Microchimica Acta. 187 |
ISSN: | 1436-5073 0026-3672 |
Popis: | To early effectively detect amyloid-beta (Aβ) oligomers, a label-free reusable aptasensor was designed. This aptasensor based on a luminescent nanoscale lanthanum-based metal-organic framework (L-MOF)-armored single-stranded DNA antibody (MOF-armored-anti-DNA antibody) as signal tags and aptamer bound to magnetic beads (Apt-MB) as capture probe. The reusable aptasensor combines signal tag and capture probe with antigen-antibody interaction. When the reusable aptasensor is formed, the strong fluorescence intensity of L-MOF will "turn off" by photo-induced electron transfer from excited states to an unfilled d shell of iron cations on the nanoparticle surface. Upon the presence of Aβ oligomers in serum samples, they can be especially distinguished with the Aβ oligomers aptamer in capture probes and then signal tags are released into the solution for developing the fluorescence aptasensor under excitation/emission 365 nm/430 nm. Meanwhile, the aptamer was recovered from the complex of Aβ oligomers/Apt-MB by heat treatment. When the temperature returns to room temperature, the recovered aptamer in the capture probe can once again bound to the MOF-armored-anti-DNA antibody for reuse. The label-free reusable aptasensor system detection has high sensitivity and selectivity toward Aβ oligomers (LOD = 0.4 pg/mL) and an excellent linear range (0.001-100 ng/mL). This strategy is a fruitful step for the development of reusable aptasensor and may turn on new avenues for the applications of Aβ oligomer detection in clinical diagnosis.Graphical abstract. |
Databáze: | OpenAIRE |
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