pat-4/ILK and unc-112/Mig-2 are required for gonad function in Caenorhabditis elegans
Autor: | Jonathan P. Miles, Xiaojian Xu, Myeongwoo Lee, Kap Duk Lee, Sharath C. Rongali |
---|---|
Rok vydání: | 2006 |
Předmět: |
Male
Ovulation endocrine system Integrin beta Chains Gonad Integrin Morphogenesis Receptors Cytoplasmic and Nuclear Protein Serine-Threonine Kinases medicine Animals Inositol 1 4 5-Trisphosphate Receptors Integrin-linked kinase Caenorhabditis elegans Caenorhabditis elegans Proteins Gonads Cytoskeleton biology urogenital system Cell migration Cell Biology biology.organism_classification Actin cytoskeleton Molecular biology Phosphoric Monoester Hydrolases Cell biology Actin Cytoskeleton Phosphotransferases (Alcohol Group Acceptor) Fertility medicine.anatomical_structure biology.protein Female RNA Interference Calcium Channels Cell Adhesion Molecules |
Zdroj: | Experimental Cell Research. 312:1475-1483 |
ISSN: | 0014-4827 |
DOI: | 10.1016/j.yexcr.2006.01.006 |
Popis: | Tissue morphogenesis requires proper interaction between cells and the extracellular matrix (ECM), which is mediated by alphabeta heterodimeric receptor integrin. In Caenorhabditis elegans, integrin signaling is essential for formation of gonad. Here, we probe the role of several integrin-associated molecules in ovulation and cell migration. Function of pat-4/integrin-linked kinase (ILK) and unc-112/Mig-2 was examined using RNA-mediated interference (RNAi). Depletion of these messages caused oocyte accumulation in the proximal gonad and distal tip cells (DTC) migration defects. It was further determined that failed ovulation was due to defective contraction and dilation of somatic gonad structures, including spermatheca and gonad sheath. Actin cytoskeleton in the proximal gonad of RNAi animals appeared disorganized, indicating that RNAi of pat-4 or unc-112 inhibited the overall assembly of actin cytoskeleton in somatic gonad. Taken together, our analysis confirms the role of integrin and integrin-associated proteins in gonad function. |
Databáze: | OpenAIRE |
Externí odkaz: |