3D Patterning of cells in Magnetic Scaffolds for Tissue Engineering

Autor: Antonio Gloria, Maurilio Marcacci, Alessandro Russo, Luigi Ambrosio, Elizaveta Kon, Y. Haranava, A. Makhaniok, Anna Tampieri, Tatiana Shelyakova, Vitaly Goranov, Valentin Dediu, R. De Santis
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Scaffold
Bone Regeneration
3d patterning
Materials science
Neovascularization
Physiologic
lcsh:Medicine
Biocompatible Materials
Nanotechnology
02 engineering and technology
Models
Biological
Proof of Concept Study
Article
03 medical and health sciences
Magnetization
Tissue engineering
Osteogenesis
Materials Testing
Human Umbilical Vein Endothelial Cells
Humans
Computer Simulation
Magnetite Nanoparticles
lcsh:Science
Multidisciplinary
Tissue Engineering
Tissue Scaffolds
lcsh:R
Mesenchymal Stem Cells
021001 nanoscience & nanotechnology
Biocompatible material
equipment and supplies
Magnetic Fields
Nanomedicine
030104 developmental biology
Models
Chemical
Cellular motility
Tissue engineering and regenerative medicine
Magnetic nanoparticles
lcsh:Q
0210 nano-technology
human activities
Zdroj: Scientific Reports, Vol 10, Iss 1, Pp 1-8 (2020)
Scientific reports (Nature Publishing Group) 10 (2020). doi:10.1038/s41598-020-58738-5
info:cnr-pdr/source/autori:Goranov V.; Shelyakova T.; De Santis R.; Haranava Y.; Makhaniok A.; Gloria A.; Tampieri A.; Russo A.; Kon E.; Marcacci M.; Ambrosio L.; Dediu V.A./titolo:3D Patterning of cells in Magnetic Scaffolds for Tissue Engineering/doi:10.1038%2Fs41598-020-58738-5/rivista:Scientific reports (Nature Publishing Group)/anno:2020/pagina_da:/pagina_a:/intervallo_pagine:/volume:10
Scientific Reports
ISSN: 2045-2322
DOI: 10.1038/s41598-020-58738-5
Popis: A three dimensional magnetic patterning of two cell types was realised in vitro inside an additive manufactured magnetic scaffold, as a conceptual precursor for the vascularised tissue. The realisation of separate arrangements of vascular and osteoprogenitor cells, labelled with biocompatible magnetic nanoparticles, was established on the opposite sides of the scaffold fibres under the effect of non-homogeneous magnetic gradients and loading magnetic configuration. The magnetisation of the scaffold amplified the guiding effects by an additional trapping of cells due to short range magnetic forces. The mathematical modelling confirmed the strong enhancement of the magnetic gradients and their particular geometrical distribution near the fibres, defining the preferential cell positioning on the micro-scale. The manipulation of cells inside suitably designed magnetic scaffolds represents a unique solution for the assembling of cellular constructs organised in biologically adequate arrangements.
Databáze: OpenAIRE
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