Nitric oxide synthase in critically ischaemic muscle and alterations in isoform expression during revascularization surgery
| Autor: | Sidney Shaw, Jcs Tsui, Michael R. Dashwood, Daryll Baker, X. Shi-Wen |
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| Rok vydání: | 2007 |
| Předmět: |
Male
Gene isoform medicine.medical_specialty Pathology Biopsy medicine.medical_treatment Blotting Western Ischemia Coronary Artery Disease Revascularization Nitric oxide chemistry.chemical_compound Internal medicine Citrulline Humans Medicine Coronary Artery Bypass Muscle Skeletal Aged Aged 80 and over Leg biology Revascularization surgery Reverse Transcriptase Polymerase Chain Reaction business.industry Middle Aged medicine.disease Immunohistochemistry Isoenzymes Nitric oxide synthase Endocrinology Bypass surgery chemistry biology.protein Female Surgery Nitric Oxide Synthase business |
| Zdroj: | British Journal of Surgery. 95:72-79 |
| ISSN: | 1365-2168 0007-1323 |
| DOI: | 10.1002/bjs.5910 |
| Popis: | Background Dysfunction of the nitric oxide pathway is implicated in peripheral arterial disease. Nitric oxide synthase (NOS) isoforms and NOS activity were studied in muscle from patients with critical leg ischaemia (CLI). Alterations in NOS during revascularization surgery were also assessed. Methods Muscle biopsies were taken from patients with CLI undergoing amputation and also from patients undergoing femorodistal bypass at the start of surgery, after arterial clamping and following reperfusion. The presence of NOS within muscle sections was confirmed using reduced nicotinamide adenine dinucleotide phosphate diaphorase histochemistry. NOS isoform distribution was studied by immunohistochemistry. NOS mRNA and protein levels were measured using real-time reverse transcriptase–polymerase chain reaction and western blotting. NOS activity was assessed with the citrulline assay. Results All three NOS isoforms were found in muscle, associated with muscle fibres and microvessels. NOS I and III protein expression was increased in CLI (P = 0·041). During revascularization, further ischaemia and reperfusion led to a rise in NOS III protein levels (P = 0·008). NOS activity was unchanged. Conclusion Alterations in NOS I and III occurred in muscle from patients with CLI and further changes occurred during bypass surgery. |
| Databáze: | OpenAIRE |
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