Kinetics of mononuclear cell infiltration and cytokine expression in iodine-induced thyroiditis in the NOD-H2h4 mouse
| Autor: | Raphael Bonita, Noel R. Rose, Patrizio Caturegli, C. Lynne Burek, Linda Rasooly |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
CD4-Positive T-Lymphocytes
endocrine system medicine.medical_specialty Pathology endocrine system diseases Clinical Biochemistry Thyroid Gland CD8-Positive T-Lymphocytes Thyroiditis Pathology and Forensic Medicine Autoimmune thyroiditis Interferon-gamma Mice Mice Inbred NOD Internal medicine medicine Animals Molecular Biology B-Lymphocytes business.industry Macrophages Thyroid Histocompatibility Antigens Class II Thyroiditis Autoimmune Antibodies Monoclonal medicine.disease Immunohistochemistry Interleukin-12 Mononuclear cell infiltration Disease Models Animal Endocrinology medicine.anatomical_structure Interleukin 12 Leukocytes Mononuclear business Infiltration (medical) CD8 Lymphocytic Thyroiditis Iodine |
| Zdroj: | Experimental and molecular pathology. 74(1) |
| ISSN: | 0014-4800 |
| Popis: | Mononuclear cell infiltration of the thyroid gland is a common histologic feature of chronic lymphocytic thyroiditis. Although the infiltrating mononuclear cells have been implicated in the destruction of the thyroid, information concerning the progression of infiltration into the thyroid is limited. In this report, we examine the composition and kinetics of mononuclear cell infiltration in the thyroid and the expression of major histocompatibility complex class II (I-Ak), IL-12, and IFN-gamma in the thyroid of the NOD-H2h4 mouse, a model of spontaneous autoimmune thyroiditis accelerated by the administration of excess dietary iodine. Mice were given a low dose of 0.015% NaI in their drinking water for 2, 4, 6, 8, and 16 weeks, and thyroids were removed, serially sectioned, and stained in an avidin-biotin peroxidase assay. The thyroid infiltrate included CD4+ and CD8+ T cells, F4/80+ macrophages, and B220+ B cells. After 2 weeks of iodine treatment, CD4+ T cells were the first seen in the thyroid, followed by CD8+ T cells and F4/80+ macrophages. B220+ B cells entered the thyroid after 4 weeks of iodine treatment. IL-12 and IFN-gamma positive cells were located in the thyroid early in disease and were up-regulated in the focal accumulations of infiltrating cells. Thyrocytes clearly expressed I-Ak after 4 weeks of iodine treatment near the location of mononuclear cell infiltration. |
| Databáze: | OpenAIRE |
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