Scavenger Receptor Collectin Placenta 1 (CL-P1) Predominantly Mediates Zymosan Phagocytosis by Human Vascular Endothelial Cells
| Autor: | Noritoshi Kitamoto, Yasuhiko Suzuki, Itsuro Yoshida, Wataru Motomura, Katsuki Ohtani, Jun Fukuzawa, Mitsuko Fukuda, Kenichiro Mori, Seongjae Jang, Nobutaka Wakamiya, Takayuki Yoshizaki, Atsushi Fukuoh |
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| Rok vydání: | 2009 |
| Předmět: |
Umbilical Veins
Antifungal Agents Cytochalasin D Cell Adhesion Molecules Neuronal Phagocytosis Collectin Mice Transgenic CHO Cells Biology Biochemistry Umbilical vein Wortmannin Mice chemistry.chemical_compound Cricetulus Cricetinae Animals Humans Scavenger receptor Molecular Biology Nucleic Acid Synthesis Inhibitors Receptors Scavenger Chinese hamster ovary cell Dextran Sulfate Zymosan Endothelial Cells Cell Biology Scavenger Receptors Class E Molecular biology Collectins Androstadienes Lipoproteins LDL Macrophage receptor with collagenous structure chemistry Poly I |
| Zdroj: | Journal of Biological Chemistry. 284:3956-3965 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m807477200 |
| Popis: | Collectin placenta 1 (CL-P1), a recently discovered scavenger receptor, mediates the uptake of oxidized low density lipoprotein and microbes. In this study, we investigated CL-P1-mediated binding and ingestion of yeast-derived zymosan bioparticles using Chinese hamster ovary (CHO) cells stably expressing human CL-P1 (CHO/CL-P1) and human vascular endothelial cells constitutively expressed CL-P1. The uptake of zymosan by CHO/CL-P1 was dependent upon the level of CL-P1 expressed on the membrane and was inhibited by cytochalasin D and wortmannin. The binding of zymosan was also inhibited by ligands of other scavenger receptors such as poly(I) and dextran sulfate. Real time reverse transcription-PCR analyses showed that other scavenger receptors, namely LOX-1, Stabilin-2, or macrophage receptor with collagenous structure (MARCO), were not expressed in human umbilical vein endothelial cells isolated from different individuals. Nonopsonic zymosan ingestion was inhibited in three primary cultured vascular endothelial cells, including different human umbilical vein endothelial cells from nine individuals treated with CL-P1 small interfering RNAs, although small interfering RNAs of other scavenger receptors had no effect on zymosan uptake in these cells. Furthermore, we confirmed that CL-P1 is expressed in human and murine vascular endothelial layers. Our results demonstrated that CL-P1 predominantly mediated phagocytosis for fungi in vascular endothelia. |
| Databáze: | OpenAIRE |
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