Lactoferrin, Quercetin, and Hydroxyapatite Act Synergistically against Pseudomonas fluorescens

Autor: Francesca Malvano, Marina Papaianni, Angela Michela Immacolata Montone, Rosanna Capparelli, Federico Capuano, Donatella Albanese
Přispěvatelé: Montone, A. M. I., Papaianni, M., Malvano, F., Capuano, F., Capparelli, R., Albanese, D.
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Anti-Infective Agent
antimicrobial additives
Food industry
Food spoilage
HA
Lacto
chemistry.chemical_compound
Nanoparticle
Anti-Infective Agents
Food science
Biology (General)
Cells
Cultured
Spectroscopy
Cultured
biology
Lactoferrin
food and beverages
Drug Synergism
U937 Cells
General Medicine
Antimicrobial
Anti-Bacterial Agents
Computer Science Applications
Chemistry
Pseudomonas fluorescens
Que complex
Dose-Response Relationship
Drug
Durapatite
Humans
Nanoparticles
Pseudomonas Infections
Quercetin
Drug
antimicrobial additive
Human
QH301-705.5
Cells
Article
Catalysis
Pseudomonas Infection
Dose-Response Relationship
Inorganic Chemistry
Functional food
Anti-Bacterial Agent
HA–Lacto–Que complex
Physical and Theoretical Chemistry
Molecular Biology
QD1-999
business.industry
Pseudomonas fluorescen
Organic Chemistry
biology.organism_classification
In vitro
chemistry
biology.protein
business
Zdroj: International Journal of Molecular Sciences, Vol 22, Iss 9247, p 9247 (2021)
International Journal of Molecular Sciences
Volume 22
Issue 17
ISSN: 1661-6596
1422-0067
Popis: Pseudomonas fluorescens is an opportunistic, psychotropic pathogen that can live in different environments, such as plant, soil, or water surfaces, and it is associated with food spoilage. Bioactive compounds can be used as antimicrobials and can be added into packaging systems. Quercetin and lactoferrin are the best candidates for the development of a complex of the two molecules absorbed on bio combability structure as hydroxyapatite. The minimum inhibiting concentration (MIC) of single components and of the complex dropped down the single MIC value against Pseudomonas fluorescens. Characterization analysis of the complex was performed by means SEM and zeta-potential analysis. Then, the synergistic activity (Csyn) of single components and the complex was calculated. Finally, the synergistic activity was confirmed, testing in vitro its anti-inflammatory activity on U937 macrophage-like human cell line. In conclusion, the peculiarity of our study consists of optimizing the specific propriety of each component: the affinity of lactoferrin for LPS
that of quercetin for the bacterial membrane. These proprieties make the complex a good candidate in food industry as antimicrobial compounds, and as functional food.
Databáze: OpenAIRE
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