Rapid mechanochemical encapsulation of biocatalysts into robust metal–organic frameworks
| Autor: | Pei En Kuo, Benjamin P. Williams, Hsun Chih Yang, Yu Shen Hsu, Wei Shang Lo, Xin Hua Chen, Zih Yin Lin, Sheng-Yu Chen, Tz Han Wei, Shi Hong Wu, Fa Kuen Shieh, Lien-Yang Chou, Yi Da Huang, Chia-Kuang Tsung |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Science
General Physics and Astronomy Nanotechnology 02 engineering and technology 010402 general chemistry 01 natural sciences Article General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound Solid substrate Enzyme Stability Cellulose lcsh:Science Multidisciplinary beta-Fructofuranosidase Strong acids beta-Glucosidase fungi General Chemistry Metal-organic frameworks Catalase Enzymes Immobilized beta-Galactosidase 021001 nanoscience & nanotechnology Enzymes 0104 chemical sciences Encapsulation (networking) chemistry Metals Biocatalysis Microscopy Electron Scanning Electrophoresis Polyacrylamide Gel Metal-organic framework lcsh:Q 0210 nano-technology Selectivity Powder Diffraction |
| Zdroj: | Nature Communications, Vol 10, Iss 1, Pp 1-8 (2019) Nature Communications |
| ISSN: | 2041-1723 |
| Popis: | Metal–organic frameworks (MOFs) have recently garnered consideration as an attractive solid substrate because the highly tunable MOF framework can not only serve as an inert host but also enhance the selectivity, stability, and/or activity of the enzymes. Herein, we demonstrate the advantages of using a mechanochemical strategy to encapsulate enzymes into robust MOFs. A range of enzymes, namely β-glucosidase, invertase, β-galactosidase, and catalase, are encapsulated in ZIF-8, UiO-66-NH2, or Zn-MOF-74 via a ball milling process. The solid-state mechanochemical strategy is rapid and minimizes the use of organic solvents and strong acids during synthesis, allowing the encapsulation of enzymes into three prototypical robust MOFs while maintaining enzymatic biological activity. The activity of encapsulated enzyme is demonstrated and shows increased resistance to proteases, even under acidic conditions. This work represents a step toward the creation of a suite of biomolecule-in-MOF composites for application in a variety of industrial processes. Metal–organic frameworks (MOFs) are attractive for encapsulating enzymes for industrial purposes because they can increase selectivity, stability, and/or activity of the enzymes. Here, the authors developed an economical solid-state mechanochemical method to encapsulate enzymes during MOF synthesis. |
| Databáze: | OpenAIRE |
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