Heterotypic recognition of recombinant FMDV proteins by bovine T-cells: the polymerase (P3Dpol) as an immunodominant T-cell immunogen
| Autor: | J Baron, T.R Doel, A Childerstone, R. M. E. Parkhouse, Mike Flint, T. Collen, Martin D. Ryan, A Corteyn, M. Garcia-Valcarcel |
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| Rok vydání: | 1998 |
| Předmět: |
CD4-Positive T-Lymphocytes
Cancer Research Immunogen viruses Protein subunit T cell Immunoblotting RNA-dependent RNA polymerase Context (language use) Cross Reactions Polymerase Chain Reaction Virus law.invention Aphthovirus Capsid law Virology medicine Animals Antigens Viral Cells Cultured biology virus diseases Viral Vaccines DNA-Directed RNA Polymerases biochemical phenomena metabolism and nutrition biology.organism_classification Infectious Diseases medicine.anatomical_structure Recombinant DNA Cattle Rabbits Foot-and-mouth disease virus |
| Zdroj: | Virus Research. 56:125-133 |
| ISSN: | 0168-1702 |
| DOI: | 10.1016/s0168-1702(98)00035-5 |
| Popis: | In this study we have examined the recognition of VP0, VP1, VP2, VP3 and P3Dpol by PBMC and CD4+ T-cells from infected, vaccinated-challenged, and multiply-vaccinated (O1, A24, C1 or ASIA1) cattle using recombinant proteins of an O1 serotype virus. The structural protein VP1 was recognised in an homotypic context whereas VP2, VP3, VP4 and P3Dpol were also recognised by T-cells from animals exposed to heterotypic viruses. Only the non-structural protein P3Dpol was consistently recognised by T-cells from the majority of animals examined and heterotypic recognition correlated with the presence of serologically detectable P3Dpol in purified virus. Thus, P3Dpol is a major cross-reactive immunodeterminant of FMDV, eliciting heterotypic T-cell responses and, therefore, with possible potential for inclusion in a subunit vaccine. |
| Databáze: | OpenAIRE |
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