Sequential chemiluminescent detection of target DNAs without stripping and reprobing
| Autor: | A P Schaap, L V Reddy, H Akhavan-Tafti, R S Handley, R DeSilva |
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| Rok vydání: | 1999 |
| Předmět: |
Genetic Markers
Genotype Cystic Fibrosis Transmembrane Conductance Regulator Horseradish peroxidase General Biochemistry Genetics and Molecular Biology law.invention chemistry.chemical_compound law Humans Horseradish Peroxidase Southern blot Chemiluminescence DNA Primers biology Base Sequence Chemistry Hybridization probe Substrate (chemistry) DNA Alkaline Phosphatase Molecular biology Blot Blotting Southern Evaluation Studies as Topic Luminescent Measurements biology.protein DNA Probes Peroxidase Biotechnology |
| Zdroj: | BioTechniques. 26(4) |
| ISSN: | 0736-6205 |
| Popis: | We present a simple method for sequential chemiluminescent detections of two different DNA loci on a single Southern blot. First, an enzyme-linked DNA probe for a unique sequence is detected with a horseradish peroxidase (HRP) substrate followed by the detection of another enzyme-linked DNA probe for a different unique sequence with an alkaline phosphatase (AP) substrate that simultaneously inhibits the chemiluminescence generated by HRP. Such sequential detection steps eliminate the need to strip and reprobe blots and can be performed with no intervening steps. |
| Databáze: | OpenAIRE |
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