The Majority of Epidermal T Cells in Psoriasis Vulgaris Lesions can Produce Type 1 Cytokines, Interferon-γ, Interleukin-2, and Tumor Necrosis Factor-α, Defining TC1 (Cytotoxic T Lymphocyte) and TH1 Effector Populations:1 a Type 1 Differentiation Bias is also Measured in Circulating Blood T Cells in Psoriatic Patients
| Autor: | Toyoko Kikuchi, Ian B. Walters, Lisa M. Austin, James G. Krueger, Maki Ozawa |
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| Rok vydání: | 1999 |
| Předmět: |
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T cell interleukin-10 Dermatology Biology Biochemistry 030207 dermatology & venereal diseases 03 medical and health sciences Interleukin 21 0302 clinical medicine medicine Cytotoxic T cell IL-2 receptor Antigen-presenting cell Molecular Biology 030304 developmental biology Interleukin 3 0303 health sciences Cell Biology Natural killer T cell intracellular 3. Good health medicine.anatomical_structure Immunology Interleukin 12 interleukin-4 |
| Zdroj: | Journal of Investigative Dermatology. 113(5):752-759 |
| ISSN: | 0022-202X |
| DOI: | 10.1046/j.1523-1747.1999.00749.x |
| Popis: | Psoriasis vulgaris is a skin disease potentially mediated by pro-inflammatory cytokines produced by type 1 lesional T cells. The capability of individual T cells to produce these cytokines in lesional skin is not known. In this study we measured the ability of lesional and peripheral blood T cells to produce intracellular interferon-γ, tumor necrosis factor-α, interleukin-2, interleukin-4, and interleukin-10 proteins as detected by flow cytometric analysis. Cytokine synthesis was induced by activation with ionomycin/phorbol myristate acetate (in the presence of Brefeldin A, which inhibits the exocytosis of these cytokines). After stimulation, we found relatively high percentages of epidermal CD8 and CD4 T cells capable of producing interferon-γ, tumor necrosis factor-α, and interleukin-2, whereas few T cells |
| Databáze: | OpenAIRE |
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