Effects of high glucose on proliferation and function of circulating fibrocytes: Involvement of CXCR4/SDF‑1 axis

Autor: Lijiang Tang, Xiaowei Liu, Senna Lin, Chenkai Xu, Yingzheng Weng, Jiangjie Lou, Changqing Du
Rok vydání: 2019
Předmět:
Blood Glucose
Male
0301 basic medicine
Benzylamines
Receptors
CXCR4
Stromal cell
proliferation
medicine.medical_treatment
Connective tissue
invasion and migration
Apoptosis
Cyclams
Peripheral blood mononuclear cell
Flow cytometry
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Cell Movement
Heterocyclic Compounds
CXC chemokine receptor 4/SDF-1
Genetics
medicine
Humans
CXC chemokine receptors
Cells
Cultured
Aged
Cell Proliferation
Connective Tissue Cells
diabetes
medicine.diagnostic_test
Growth factor
circulating fibrocytes
fibrosis
Articles
General Medicine
Middle Aged
Molecular biology
Chemokine CXCL12
CTGF
Glucose
030104 developmental biology
medicine.anatomical_structure
Diabetes Mellitus
Type 2
Gene Expression Regulation
L-Glucose
chemistry
030220 oncology & carcinogenesis
Female
Signal Transduction
Zdroj: International Journal of Molecular Medicine
ISSN: 1791-244X
1107-3756
Popis: The present study aimed to further investigate the effects of high glucose on the function of circulating fibrocytes and its underlying mechanisms. The total peripheral blood mononuclear cells were obtained from normal glucose tolerance patients and type 2 diabetic mellitus patients. Circulating fibrocytes were stimulated with different glucose concentrations for different time periods (24, 48 and 72 h). Cell proliferation was determined by Cell Counting Kit-8 assay. The expression of connective tissue growth factor (CTGF) was detected by western blotting. The expression of COL-I was detected by flow cytometry. The apoptotic bodies of cells were detected by fluorescence microscopy after Hoechst33258 staining. The invasive and migration abilities of fibrocytes were detected by Transwell chamber assay. Secretion of stromal cell-derived factor 1 (SDF-1) was measured by ELISA. The circulating fibrocytes showed a typical spindle-shape and were double-positive for cluster of differentiation 45 (green) and COL-I (red). Compared with the 5.5 mmol/l glucose group, a high glucose concentration significantly promoted the proliferation of circulating fibrocytes and showed the most significant effects at 30 mmol/l after treatment for 48 h. AMD3100 showed no effects on the proliferation of circulating fibrocytes. Flow cytometry revealed that 30 mmol/l glucose significantly promoted the expression of COL-I vs. 5.5 mmol/l glucose group (P0.05). Western blotting revealed that the expression of CTGF was decreased significantly by AMD3100 pretreatment (P0.05). High glucose stimulated the expressions of CTGF and COL-I, and promoted migration of circulating fibrocytes via the CXC chemokine receptor 4/SDF-1 axis.
Databáze: OpenAIRE
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