Chromosome instability predicts progression of premalignant lesions of the larynx
| Autor: | Ernst-Jan M. Speel, Michelene N. Chenault, Sophie G.H. Litjens, Jacqueline M. Van der Wal, Adri C. Voogd, Verona E. Bergshoeff, Fredrik J. Bot, Ed Schuuring, Frans C. S. Ramaekers, Annick Haesevoets, Stijn J.A. Van der Heijden, Johannes J. Manni, Bernd Kremer, Anton H. N. Hopman |
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| Přispěvatelé: | Damage and Repair in Cancer Development and Cancer Treatment (DARE), Targeted Gynaecologic Oncology (TARGON), Pathologie, Epidemiologie, FHML Methodologie & Statistiek, Moleculaire Celbiologie, Keel-, Neus- en Oorheelkunde, KNO, RS: CAPHRI School for Public Health and Primary Care, RS: GROW - R1 - Prevention, RS: GROW - R3 - Innovative Cancer Diagnostics & Therapy, RS: GROW - R2 - Basic and Translational Cancer Biology, RS: CAPHRI - Clinical epidemiology, RS: CAPHRI - Design and analysis of studies in health sciences, RS: GROW - Oncology, RS: MHeNs - R3 - Neuroscience, Genetica & Celbiologie, Interne Geneeskunde, MUMC+: MA Medische Oncologie (9) |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Adult
Male p53 EXPRESSION medicine.medical_specialty Pathology MICROSATELLITE INSTABILITY Fas-Associated Death Domain Protein cyclin D1 MALIGNANT ORAL LESIONS Kaplan-Meier Estimate Biology premalignant Disease-Free Survival Pathology and Forensic Medicine Malignant transformation head and neck Young Adult FISH NECK-CANCER Chromosomal Instability Chromosome instability medicine RESECTION MARGINS Humans HEAD DYSPLASIA Laryngeal Neoplasms In Situ Hybridization Fluorescence Aged Moderate Dysplasia larynx Polysomy Hyperplasia Carcinoma in situ Microsatellite instability Middle Aged medicine.disease Dysplasia FADD Disease Progression Female Histopathology SQUAMOUS-CELL CARCINOMA Precancerous Conditions |
| Zdroj: | Pathology, 46(3), 216-224 Pathology, 46(3), 216-224. LIPPINCOTT WILLIAMS & WILKINS |
| ISSN: | 0031-3025 |
| Popis: | Summary The histopathology of premalignant laryngeal lesions does not provide reliable information on the risk of malignant transformation, hence we examined new molecular markers which can easily be implemented in clinical practice. Dual-target fluorescence in situ hybridisation (FISH) for chromosome 1 and 7 centromeres was performed on tissue sections of laryngeal premalignancies in 69 patients. Chromosome instability was indicated by numerical imbalances and/or polysomy for chromosomes 1 and 7. Additionally immuno-stainings for p53, Cyclin D1 and (p)FADD expression were evaluated. Malignant progression was recorded. Eighteen patients with carcinoma in situ (CIS) were treated after diagnosis and excluded from follow-up. Chromosome instability was strongly associated with a high risk of malignant transformation, especially in lower grade lesions (hyperplasia, mild and moderate dysplasia; odds ratio = 8.4, p = 0.004). Patients with lesions containing chromosome instability showed a significantly worse 5-year progression-free survival than those with premalignancies without chromosome instability ( p = 0.002). Neither histopathology nor the protein markers predicted progression in univariate analysis, although histo-pathological diagnosis, p53 and FADD contributed positively to chromosome instability in multivariate analysis. Chromosome instability is associated with malignant progression of laryngeal premalignancies, especially in lower grade lesions. These results may contribute to better risk counselling, provided that they can be validated in a larger patient set. |
| Databáze: | OpenAIRE |
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