LIPOSOME-MEDIATED INTRAPERITONEAL INTERLEUKIN 10 GENE TRANSFER INCREASES SURVIVAL IN CECAL LITIGATION AND PUNCTURE MODEL OF SEPSIS
Autor: | Meral Özgüç, Atac Baykal, Y. Çetin Kocaefe, Iskender Sayek, Burhan Kabay |
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Rok vydání: | 2006 |
Předmět: |
Endothelium
medicine.medical_treatment Transgene Critical Care and Intensive Care Medicine law.invention Sepsis Mice law Intensive care medicine Animals Humans Mice Inbred BALB C business.industry Gene Transfer Techniques Genetic Therapy medicine.disease Recombinant Proteins Interleukin-10 Disease Models Animal Interleukin 10 medicine.anatomical_structure Cytokine Tolerability Liposomes Immunology Emergency Medicine Recombinant DNA business |
Zdroj: | Shock. 26:37-40 |
ISSN: | 1073-2322 |
Popis: | Interleukin 10 (IL-10) has been considered to alleviate the inflammatory cytokine response in various models of sepsis. Although being regarded as a key immunomodulator molecule to be beneficial for the treatment of sepsis, recombinant IL-10 treatment is limited by efficacy and tolerability. We tested a novel approach and conducted i.p. liposomal IL-10 gene transfer 24 h before the cecal ligation and puncture in mice and observed 75% mortality at the end of the 7th day. The mortality was 100% in the group where the gene transfer was not performed. The transgene expression is observed mainly in the endothelium in all vital organs. The results demonstrate the advantageous role of de novo IL-10 synthesis in early stages of sepsis and suggest the beneficial impact of gene transfer approach to recombinant protein infusions. |
Databáze: | OpenAIRE |
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