On the involvement of inorganic ions in the effect of gamma-aminobutyric acid on brain serotonin and norepinephrine
| Autor: | E. K. Kazarova, N. H. Yessaian, H. Ch. Buniatian, A. R. Armenian |
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| Rok vydání: | 1971 |
| Předmět: |
medicine.medical_specialty
Serotonin Brain Monoamines Inorganic ions In Vitro Techniques Biochemistry gamma-Aminobutyric acid Ouabain Norepinephrine (medication) Midbrain Cellular and Molecular Neuroscience Norepinephrine Mesencephalon Internal medicine medicine Animals Fluorometry Incubation Chemistry Aminobutyrates Sodium Water-Electrolyte Balance Rats Endocrinology nervous system Spectrophotometry Potassium Calcium medicine.drug |
| Zdroj: | Journal of neurochemistry. 18(3) |
| ISSN: | 0022-3042 |
| Popis: | — The loss of GABA, norepinephrine and serotonin and the uptake of GABA (in the presence of 1 mM-GABA) and the effect of GABA on the loss of norepinephrine and serotonin were investigated in rat midbrain slices incubated in media of various compositions. In a medium of low Na+ concentration the loss of serotonin from incubated slices was markedly inhibited while that of norepinephrine and GABA was significantly increased. Conversely the most pronounced loss of serotonin from slices was observed on the addition of ouabain to a medium of a balanced ionic composition. Whereas the loss of serotonin from slices increased in a medium of high K+ content, it was significantly reduced after 45 min incubation in a high K+-low Na+ medium. In all the modified media used, a significant loss of norepinephrine was observed while that of GABA was not affected by the omission of Ca2+ and was slightly reduced in the absence of K+. GABA enhanced the loss of norepinephrine and inhibited that of serotonin in a high-K+ medium and in one with a balanced ionic composition. A deficiency of Na+ in the medium had a differential effect on the loss of norepinephrine and serotonin similar to that observed with 1 mM-GABA. These results suggest that Na+ may be of crucial importance in the release of serotonin from midbrain slices and that an enhancement of the Na+ extrusion mechanism at the synaptosomal level may be involved in the effect of GABA on brain monoamines. |
| Databáze: | OpenAIRE |
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