Staphylococcus aureus Skin Colonization Is Enhanced by the Interaction of Neutrophil Extracellular Traps with Keratinocytes
| Autor: | Jule Focken, Laura Klink, Zsofia Bittner, Martin Schaller, Lena Staudenmaier, Franziska Herster, Lisa Bleul, Birgit Schittek, Christiane Wolz, Andreas Peschel, Birgit Fehrenbacher, Birgit Sauer, Katharina Bitschar, Alexander N.R. Weber |
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| Rok vydání: | 2020 |
| Předmět: |
Keratinocytes
Male 0301 basic medicine Staphylococcus aureus Cell signaling Neutrophils Inflammation Cell Communication Dermatology Biology medicine.disease_cause Extracellular Traps Biochemistry Dermatitis Atopic Microbiology Mice 03 medical and health sciences 0302 clinical medicine medicine Animals Humans Colonization Surgical Tape Molecular Biology Pathogen Cells Cultured Skin Skin protection integumentary system Microbiota Cell Biology Atopic dermatitis Neutrophil extracellular traps Staphylococcal Infections medicine.disease Coculture Techniques 030104 developmental biology 030220 oncology & carcinogenesis Female medicine.symptom |
| Zdroj: | Journal of Investigative Dermatology. 140:1054-1065.e4 |
| ISSN: | 0022-202X |
| DOI: | 10.1016/j.jid.2019.10.017 |
| Popis: | Staphylococcus aureus is a facultative pathogen found on skin and nasal surfaces. It is usually absent from the skin of healthy humans but frequently colonizes the skin of patients with atopic dermatitis. Here, we investigate the functional role of neutrophils in the initial steps of S. aureus skin colonization and how skin commensals modulate the S. aureus-induced recruitment of neutrophils to the skin. Using an epicutaneous mouse skin colonization model, we show that skin inflammation induced by tape-stripping leads to a rapid recruitment of neutrophils, which correlates with enhanced S. aureus skin colonization. Interestingly, the depletion of neutrophils in vivo reduces S. aureus colonization, and in vitro coculture of primary human keratinocytes with neutrophils promotes S. aureus adherence. We demonstrate that the interaction of neutrophil extracellular traps with keratinocytes are responsible for the increased S. aureus skin colonization. Finally, we show that S. epidermidis as part of the skin microbiota can reduce the neutrophil recruitment induced by S. aureus infection. These data suggest that microbiota-mediated skin protection against S. aureus is dampened in an inflammatory environment in which neutrophil extracellular traps released by infiltrating neutrophils unexpectedly contribute to enhanced S. aureus skin colonization. |
| Databáze: | OpenAIRE |
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