Proteomic analysis and interactions network in leaves of mycorrhizal and nonmycorrhizal sorghum plants under water deficit
| Autor: | Armando Guerrero-Rangel, Silvia Valdés-Rodríguez, Argel Gastelum-Arellanez, Robert Winkler, Víctor Olalde-Portugal, José Luis Cabrera-Ponce |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Drought tolerance Ribosomal proteins Sulfur metabolism Sulfurtransferase Proteomic analysis Arbuscular mycorrhizal fungi lcsh:Medicine Plant Science Photosynthesis 01 natural sciences Malate dehydrogenase Biochemistry General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Ribosomal protein Botany Agricultural Science Molecular Biology Sorghum Water deficit 030304 developmental biology 0303 health sciences biology General Neuroscience fungi lcsh:R food and beverages General Medicine Gene network biology.organism_classification Signaling Protein–protein interaction Metabolic pathway ATP synthase General Agricultural and Biological Sciences 010606 plant biology & botany Biotechnology |
| Zdroj: | PeerJ, Vol 8, p e8991 (2020) PeerJ |
| ISSN: | 2167-8359 |
| Popis: | For understanding the water deficit stress mechanism in sorghum, we conducted a physiological and proteomic analysis in the leaves of Sorghum bicolor L. Moench (a drought tolerant crop model) of non-colonized and colonized plants with a consortium of arbuscular mycorrhizal fungi. Physiological results indicate that mycorrhizal fungi association enhances growth and photosynthesis in plants, under normal and water deficit conditions. 2D-electrophoresis profiles revealed 51 differentially accumulated proteins in response to water deficit, of which HPLC/MS successfully identified 49. Bioinformatics analysis of protein–protein interactions revealed the participation of different metabolic pathways in nonmycorrhizal compared to mycorrhizal sorghum plants under water deficit. In noninoculated plants, the altered proteins are related to protein synthesis and folding (50S ribosomal protein L1, 30S ribosomal protein S10, Nascent polypeptide-associated complex subunit alpha), coupled with multiple signal transduction pathways, guanine nucleotide-binding beta subunit (Rack1) and peptidyl-prolyl-cis-trans isomerase (ROC4). In contrast, in mycorrhizal plants, proteins related to energy metabolism (ATP synthase-24kDa, ATP synthase β), carbon metabolism (malate dehydrogenase, triosephosphate isomerase, sucrose-phosphatase), oxidative phosphorylation (mitochondrial-processing peptidase) and sulfur metabolism (thiosulfate/3-mercaptopyruvate sulfurtransferase) were found. Our results provide a set of proteins of different metabolic pathways involved in water deficit produced by sorghum plants alone or associated with a consortium of arbuscular mycorrhizal fungi isolated from the tropical rain forest Los Tuxtlas Veracruz, México. |
| Databáze: | OpenAIRE |
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