Laryngeal transplantation in minipigs: early immunological outcomes
| Autor: | MA Birchall, Alan Jones, Sarah M. Ayling, Rachel Burt, PJ Murison, Paolo Macchiarini, Mick Bailey, LK Mitchard, Christopher R. Stokes, Ross Harley |
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| Rok vydání: | 2012 |
| Předmět: |
Male
Pathology medicine.medical_specialty Time Factors Swine medicine.medical_treatment Immunology Major histocompatibility complex Major Histocompatibility Complex Antigen Antigens CD Transplantation Immunology medicine Immunology and Allergy Animals Transplantation Homologous IL-2 receptor Subglottis biology T-cell receptor Animal Models Transplantation Laryngectomy Genes T-Cell Receptor beta Models Animal biology.protein Swine Miniature Female Larynx CD8 |
| Zdroj: | Clinical and experimental immunology. 167(3) |
| ISSN: | 1365-2249 |
| Popis: | Summary Despite recent tissue-engineering advances, there is no effective way of replacing all the functions of the larynx in those requiring laryngectomy. A recent clinical transplant was a success. Using quantitative immunofluorescence targeted at immunologically relevant molecules, we have studied the early (48 h and 1 week) immunological responses within larynxes transplantated between seven pairs of National Institutes of Health (NIH) minipigs fully homozygous at the major histocompatibility complex (MHC) locus. There were only small changes in expression of some molecules (relative to interindividual variation) and these were clearest in samples from the subglottic region, where the areas of co-expression of CD25+CD45RC-CD8- and of CD163+CD172+MHC-II- increased at 1 week after transplant. In one case, infiltration by recipient T cells was analysed by T cell receptor (TCR) Vβ spectratype analysis; this suggested that changes in the T cell repertoire occur in the donor subglottis mucosal tissues from day 0 to day 7, but that the donor and recipient mucosal Vβ repertoires remain distinct. The observed lack of strong immunological responses to the trauma of surgery and ischaemia provides encouraging evidence to support clinical trials of laryngeal transplantation, and a basis on which to interpret future studies involving mismatches. |
| Databáze: | OpenAIRE |
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