Secondary Structure and Tertiary Fold of the Birch Pollen Allergen Bet v 1 in Solution
| Autor: | Andreas Kungl, Michael Breitenbach, Dietrich Kraft, Markus Susani, Cornelius Faber, Rainer Frank, Almut Lindemann, Andrzej Ejchart, Heinrich Sticht, Paul Rösch |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Protein Folding
Magnetic Resonance Spectroscopy Molecular Sequence Data Peptide Antiparallel (biochemistry) Biochemistry Protein Structure Secondary Escherichia coli Organic chemistry Amino Acid Sequence Cloning Molecular Molecular Biology Protein secondary structure Plant Proteins chemistry.chemical_classification Molecular mass Chemistry C-terminus Cell Biology Nuclear magnetic resonance spectroscopy Allergens Antigens Plant Protein tertiary structure Protein Structure Tertiary Solutions Crystallography Heteronuclear molecule |
| Zdroj: | Journal of Biological Chemistry. 271:19243-19250 |
| ISSN: | 0021-9258 |
| Popis: | Bet v 1 is the major birch pollen allergen and therefore the main cause of type I allergies observed in early spring. It is composed of 159 amino acid residues adding up to a molecular mass of 17 kDa. We determined the secondary structure and tertiary fold of full-length Bet v 1 by NMR spectroscopy. Two- and three-dimensional NMR measurements suggest that Bet v 1 is a globular monomer in solution with a high content of well defined secondary structure. Of the total of 159 residues, 135 could be sequentially assigned, using an improved assignment strategy based mainly on heteronuclear experiments. An improved strategy for structure calculation revealed three helices and two beta-sheets as major elements of secondary structure. The globular tertiary structure is mainly stabilized by two antiparallel beta-sheets. The two helices at the C terminus are in accordance with the results from the solution structure of the chemically synthesized peptide Bet v 1-(125-154). This peptide is composed of two helices connected by a hinge. The structural features of Bet v 1 are highly similar to those found in the Ambrosia allergen Amb t V. |
| Databáze: | OpenAIRE |
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