Nuclear proteins from HeLa cells are immunoreactive with anti-yeast Yp20 ras related antibodies
| Autor: | Valerie Stanik, Channa Shalitin |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Biophysics
Cross Reactions Biochemistry Fungal Proteins HeLa chemistry.chemical_compound Immunoblot Analysis Humans Electrophoresis Gel Two-Dimensional Nuclear protein Molecular Biology Antibodies Fungal biology Nuclear Proteins Cell Biology biology.organism_classification Molecular biology Yeast Nucleosomes Chromatin chemistry ras Proteins biology.protein PMSF DNA HeLa Cells Micrococcal nuclease |
| Zdroj: | Biochemical and Biophysical Research Communications. 168:580-588 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(90)92360-c |
| Popis: | Immunoblot analysis using antibody specific to yeast ras-related protein Yp20 revealed a 21kDa protein associated with HeLa cell chromatin which immunologically cross-reacted with this ras-related protein. The 21kDa protein can be rapidly released from the HeLa nuclei by micrococcal nuclease digestion. It seems to be associated with long mononucleosomes as determined by separation of chromatin subunits on sucrose gradients. We suggest that the 21kDa HeLa protein may be the human homologue of yeast Yp20. |
| Databáze: | OpenAIRE |
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