Western blot analysis of the antigen in pemphigoid gestationis
| Autor: | Irene M. Leigh, Balbir S. Bhogal, Susan E. Kelly, P. Whitehead, Fenella Wojnarowska, Martin M. Black |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Dystonin
Blotting Western Nerve Tissue Proteins Dermatology Autoantigens Antigen Western blot Bullous Pemphigoid Antigen immune system diseases Pemphigoid Gestationis Pemphigoid Bullous Medicine Humans skin and connective tissue diseases Skin medicine.diagnostic_test integumentary system Skin Diseases Vesiculobullous business.industry Non-Fibrillar Collagens medicine.disease eye diseases Cytoskeletal Proteins Immunology Bullous pemphigoid Collagen business Normal skin Carrier Proteins |
| Popis: | SUMMARY Using an immunoblotting technique, sera from 25 patients with pemphigoid gestationis were examined and tested against epidermal and dermal extracts of normal skin. The major antigen recognized by seven patients'sera was a molecule of 180 kDa, pemphigoid gestationis antigen, extractable only from the epidermis. Sera from 18 patients with bullous pemphigoid were studied as positive controls and the major antigen recognized was a larger molecule of 220 kDa. There was some degree of shared recognition of antigens with three patients with pemphigoid gestationis recognizing the 220 kDa bullous pemphigoid antigen. In addition one bullous pemphigoid serum recognized the 180 kDa pemphigoid gestationis antigen. The dominant pemphigoid gestationis antigen, however, differs from bullous pemphigoid antigen. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|