Accurate quantitation of standard peptides used for quantitative proteomics

Autor:	David G. Klapper, Narisa K. Bordeerat, Leonard B. Collins, James A. Swenberg, Gunnar Boysen, Nadia I. Georgieva, Christoph H. Borchers, Tyra J. Cross
Jazyk:	angličtina
Rok vydání:	2009
Předmět:	Proteomics Chromatography Chemistry Quantitative proteomics Chromatography liquid Mass spectrometry Tandem mass spectrometry Biochemistry Sensitivity and Specificity Article Tandem Mass Spectrometry Peptides Molecular Biology Quantitative analysis (chemistry) Chromatography Liquid
Popis:	Mass spectrometry-based proteomics has become an indispensable tool in system biology generating a need for accurate and precise quantitation of peptide standards. The presented method utilizes ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) to accurately quantify peptide standards at concentrations of 0.1-10 μM. The ability for accurate quantitation of micro-molar concentrations has the advantages that quantitation can be performed routinely with high precision and the high sensitivity of the method minimizes the amounts required.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1cf4580991e91fa079543d86c783f940 https://europepmc.org/articles/PMC2766299/ Zobrazit plný text záznamu