Determination of conjugated and unconjugated serum 3 alpha-OH bile acids by high-performance liquid chromatography
| Autor: | K.J. Gaskin, J W Earl, R F Goldsmith, M Gruca, M T O'Halloran |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
030213 general clinical medicine
Fluorophore 3-Hydroxysteroid Dehydrogenases medicine.drug_class Clinical Biochemistry 030209 endocrinology & metabolism Fractionation Buffers High-performance liquid chromatography Bile Acids and Salts 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Humans Solid phase extraction Derivatization Chromatography High Pressure Liquid Fluorescent Dyes Detection limit Dansyl Compounds Chromatography Bile acid Hydrolysis Reproducibility of Results General Medicine Reference Standards 3-alpha-Hydroxysteroid Dehydrogenase (B-Specific) Hydrazines chemistry Quantitative analysis (chemistry) Oxidation-Reduction |
| Zdroj: | Annals of clinical biochemistry. 31 |
| ISSN: | 0004-5632 |
| Popis: | A simple reverse-phase liquid chromatographic method has been developed for the quantitative measurement of 11 3α-OH bile acids in paediatric serum samples. Bile acids are enzymatically reduced to the corresponding 3-keto compound and then derivatized with the fluorophore dansyl hydrazine. Preliminary fractionation of bile acids is not required. The limit of detection is 0·1μmol/L using a sample size of 200μL. One hundred and twenty-three serum samples were analysed by the high-performance liquid chromatography (HPLC) method and the results compared with a commercial kit method (Enzabile) presently used in many laboratories for the measurement of total bile acids. There was a good correlation between the two methods ( r = 0·96). The method is suitable for use in a clinical laboratory for the further investigation of those patients with abnormally high total bile acid levels where quantification of bile acid fractions is required. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|