Silver enhancement of protein A-gold probes on resin-embedded ultrathin sections
| Autor: | A. R. Tognetti, Antonio Marchetti, M. Bistocchi |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Silver
Histology law.invention Metal Mice chemistry.chemical_compound law Animals Antigens Viral Tumor Staphylococcal Protein A Molecular Biology Cells Cultured Mice Inbred BALB C Staining and Labeling biology Mouse mammary tumor virus Mammary Neoplasms Experimental Cell Biology General Medicine biology.organism_classification Photographic developer Staining Microscopy Electron Medical Laboratory Technology Silver nitrate Mammary Tumor Virus Mouse chemistry visual_art Immunologic Techniques visual_art.visual_art_medium Biophysics biology.protein Gold Anatomy Electron microscope General Agricultural and Biological Sciences Protein A Immunostaining |
| Zdroj: | Histochemistry. 86:371-373 |
| ISSN: | 1432-119X 0301-5564 |
| Popis: | A simple method is described allowing the enhancement of the visibility of small gold probes for the electron microscopy. This method, which allows the silver intensification of gold directly on epon-embedded ultrathin sections, was used for the electron microscopic localization of Mouse Mammary Tumor Virus (MMTV) antigens in cultured cells derived from GR and BALB/cfRIII mouse mammary tumors. After the immunostaining with the preembedding protein A-gold technique, the ultrathin sections, placed on 200 mesh copper grids, were rehydrated and exposed to a photographic developer containing silver nitrate. During this physical development gold particles are incapsulated in growing shells of metallic silver, which gradually become more and more visible. We were able to obtain a heavy labelling of the viral particles, well visible even at low magnification, with a negligeable background staining. The present technique can be useful whenever it is necessary to use the smallest gold probes today available. |
| Databáze: | OpenAIRE |
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