Flow cytometric analysis of CK19 expression in the peripheral blood of breast carcinoma patients: relevance for circulating tumor cell detection
| Autor: | Haiming Wei, Min Cheng, Xu Wu, Yajing Liu, Yanyan Wang, Lili Wang |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Adult
CA15-3 Cancer Research Pathology medicine.medical_specialty Indicator Dilution Techniques Antineoplastic Agents Breast Neoplasms Sensitivity and Specificity lcsh:RC254-282 Benign tumor Flow cytometry 03 medical and health sciences 0302 clinical medicine Breast cancer Circulating tumor cell Cell Line Tumor Humans Medicine Aged Neoplasm Staging 030304 developmental biology Aged 80 and over Keratin-19 0303 health sciences medicine.diagnostic_test business.industry Research Middle Aged Flow Cytometry Neoplastic Cells Circulating lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens medicine.disease 3. Good health Oncology 030220 oncology & carcinogenesis Cancer cell business Breast carcinoma A431 cells |
| Zdroj: | Journal of Experimental & Clinical Cancer Research; Vol 28 Journal of Experimental & Clinical Cancer Research, Vol 28, Iss 1, p 57 (2009) Journal of Experimental & Clinical Cancer Research Journal of Experimental & Clinical Cancer Research : CR |
| ISSN: | 1756-9966 |
| DOI: | 10.1186/1756-9966-28-57 |
| Popis: | Background Immunocytochemistry and RT-PCR have been widely used for the detection of circulating tumor cells in patients with breast cancer but their specificity is limited. Our purpose is to utilize a convenient and specific technology to detect circulating tumor cells in breast cancer patients. Methods To determine the sensitivity and specificity of our method, A431 cells were serially diluted with human peripheral blood leukocytes and stained with CK19. A total of 73 blood specimens including 25 healthy volunteers and 48 patients with breast carcinoma and benign tumor were tested by flow cytometry to quantify the expression of CK19. Results The detectable upper limit of A431 cells was 1 cancer cell among 104 human white blood cells. CK19 was detected in 27% of breast cancer patients but none control gives positive result. The number of cancer cells increased gradually along with the disease stages for it was the least in stage I (0%) and the most in stage IV (1.29%). Fifteen patients were observed during three month chemotherapy after surgery, and most of their CK19 expression levels declined after treatment. Conclusion Our research convinces that the detection of CK19 in peripheral blood by flow cytometry is also a specific and feasible method to monitor circulating tumor cells in breast cancer. |
| Databáze: | OpenAIRE |
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