Tumor necrosis factor α-308 gene variants in relation to major histocompatibility complex alleles and Felty's syndrome
| Autor: | Eric L. Kaijzel, Cornelis L. Verweij, Albert Naipal, Marius J. Giphart, Brigitta M. N. Brinkman, Ferdinand C. Breedveld, Mohamed R. Daha, Anneke Verhoef |
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| Rok vydání: | 1994 |
| Předmět: |
Molecular Sequence Data
Immunology Major histocompatibility complex Major Histocompatibility Complex chemistry.chemical_compound Genotype medicine Humans Immunology and Allergy Allele Lymphotoxin-alpha Gene Alleles Base Sequence biology Tumor Necrosis Factor-alpha General Medicine medicine.disease Molecular biology Felty's syndrome chemistry Felty Syndrome biology.protein Tumor necrosis factor alpha Restriction fragment length polymorphism DNA |
| Zdroj: | Human Immunology. 41:259-266 |
| ISSN: | 0198-8859 |
| Popis: | The location of the human TNF genes within the MHC complex has prompted much speculation about the role of TNF alleles in the etiology of MHC-associated autoimmune diseases. On sequencing the 5′ regulatory region of the human TNFA gene a G ( TNFA −308G ) to A ( TNFA −308A ) transition polymorphism at position −308 was discovered. We have developed a simple PCR assay to facilitate the screening of the −308 polymorphism at the DNA level. In view of the possible linkage between the TNFA −308A allele and a certain MHC type, TNFA −308 genotypes in HLA-typed healthy individuals ( n = 88) were determined. A statistically significant association between the TNFA −308A allele and HLA-DR3, DQB1 ∗ 0201, DQA1 ∗ 0501, A1, B8, and the Ncol 5.5-kb RFLP of the TNFB gene was observed. In addition, we determined the frequency of the TNFA −308A allele in patients with FS ( n = 13), an HLA-DR4-associated disease. In this study, no association was found of Felty's syndrome with the TNFA −308A allele, indicating that this allele does not appear to be a susceptibility factor for FS. Human Immunology 41, 259–266 (1994) . |
| Databáze: | OpenAIRE |
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