C57BL/6J mice as a polygenic developmental model of diet-induced obesity
Autor: | Dinh-Toi Chu, Leslie P. Kozak, Elzbieta Malinowska, Magdalena Jura |
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Rok vydání: | 2017 |
Předmět: |
Male
0301 basic medicine medicine.medical_specialty Physiology diet‐induced obesity Gene Expression Mice 03 medical and health sciences Physiology (medical) Internal medicine Genetic model Gene expression Metabolism and Regulation epigenetic mechanisms of obesity medicine Animals Obesity Promoter Regions Genetic Gene Adaptor Proteins Signal Transducing Adiposity Nutrition Original Research biology Microarray analysis techniques Developmental control of obesity DNA Methylation medicine.disease Dietary Fats Diet Mice Inbred C57BL Disease Models Animal lipogenic genes 030104 developmental biology Endocrinology Histone Adipose Tissue Lipogenesis DNA methylation biology.protein Intercellular Signaling Peptides and Proteins genetic variation of obesity Adipose Tissue and Obesity |
Zdroj: | Physiological Reports |
ISSN: | 2051-817X |
Popis: | Susceptibility to obesity changes during the course of life. We utilized the C57BL/6J (B6) and 129S mouse as a genetic model for variation in diet‐induced obesity to define the adiposity phenotypes from birth to maturity at 8 weeks‐of‐age. From birth to 8 weeks‐of‐age, both male and female 129S mice had significantly higher fat mass and adiposity index than B6 mice, although they were not obese. After 8 weeks‐of‐age, B6 had greater adiposity/obesity than 129S mice in response to a high fat (HF). We sought to determine the mechanism activating the fat accumulation in B6 mice at 8‐weeks‐of‐age. We used microarray analysis of gene expression during development of inguinal fat to show that molecular networks of lipogenesis were maximally expressed at 8 weeks‐of‐age. In addition, the DNA methylation analysis of the Sfrp5 promoter and binding of acetylated histones to Sfrp5 and Acly promoter regions showed that major differences in the expression of genes of lipogenesis and chromatin structure occur during development. Differences in lipogenesis networks could account for the strain‐dependent differences in adiposity up to 8 weeks‐of‐age; however, changes in the expression of genes in these networks were not associated with the susceptibility to DIO in B6 male mice beyond 8 weeks‐of‐age. |
Databáze: | OpenAIRE |
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