Fabrication of Kidney Proximal Tubule Grafts Using Biofunctionalized Electrospun Polymer Scaffolds

Autor: Jansen, Katja, Castilho, Miguel, Aarts, Sanne, Kaminski, Michael M, Lienkamp, Soeren S, Pichler, Roman, Malda, Jos, Vermonden, Tina, Jansen, Jitske, Masereeuw, Rosalinde, Afd Pharmacology, LS Equine Muscoskeletal Biology, Afd Pharmaceutics, Pharmacology, Pharmaceutics, dES RMSC
Přispěvatelé: Afd Pharmacology, LS Equine Muscoskeletal Biology, Afd Pharmaceutics, Pharmacology, Pharmaceutics, dES RMSC, Orthopaedic Biomechanics, EAISI Health
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Kidney Failure
Chronic/surgery
Biocompatible Materials/therapeutic use
business.product_category
Polymers and Plastics
Polymers
Transplants
Biocompatible Materials
renal transport
02 engineering and technology
SDG 3 – Goede gezondheid en welzijn
01 natural sciences
Regenerative medicine
Kidney Tubules
Proximal
Kidney Failure
Lactones
chemistry.chemical_compound
Tissue engineering
Taverne
Materials Chemistry
Tissue Engineering/methods
Proximal/cytology
Caproates/chemistry
Cells
Cultured
Kidney
Cultured
Tissue Scaffolds
021001 nanoscience & nanotechnology
Electrospinning
Kidney Tubules
medicine.anatomical_structure
Tubule
Lactones/chemistry
tissue engineering
Polycaprolactone
Transplants/growth & development
0210 nano-technology
renal replacement therapy
Biotechnology
Cells
regenerative medicine
Bioengineering
010402 general chemistry
Article
Epithelial Cells/cytology
Chronic/surgery
Biomaterials
All institutes and research themes of the Radboud University Medical Center
SDG 3 - Good Health and Well-being
polycaprolactone
Microfiber
medicine
Journal Article
Humans
Kidney Tubules
Proximal/cytology
Caproates
Cell Proliferation
Epithelial Cells
0104 chemical sciences
Renal disorders Radboud Institute for Molecular Life Sciences [Radboudumc 11]
chemistry
Nanofiber
Kidney Failure
Chronic
business
Biomedical engineering
Zdroj: Macromolecular Bioscience, 19(2). Wiley-VCH Verlag
Macromolecular Bioscience, 19(2):1800412. Wiley-VCH Verlag
Macromolecular Bioscience, 19, 2
Macromolecular Bioscience
Macromolecular Bioscience, 19
Macromolecular bioscience
ISSN: 1616-5187
Popis: The increasing prevalence of end-stage renal disease and persistent shortage of donor organs call for alternative therapies for kidney patients. Dialysis remains an inferior treatment as clearance of large and protein-bound waste products depends on active tubular secretion. Biofabricated tissues could make a valuable contribution, but kidneys are highly intricate and multifunctional organs. Depending on the therapeutic objective, suitable cell sources and scaffolds must be selected. This study provides a proof-of-concept for stand-alone kidney tubule grafts with suitable mechanical properties for future implantation purposes. Porous tubular nanofiber scaffolds are fabricated by electrospinning 12%, 16%, and 20% poly-ε-caprolactone (PCL) v/w (chloroform and dimethylformamide, 1:3) around 0.7 mm needle templates. The resulting scaffolds consist of 92%, 69%, and 54% nanofibers compared to microfibers, respectively. After biofunctionalization with L-3,4-dihydroxyphenylalanine and collagen IV, 10 × 10 6 proximal tubule cells per mL are injected and cultured until experimental readout. A human-derived cell model can bridge all fiber-to-fiber distances to form a monolayer, whereas small-sized murine cells form monolayers on dense nanofiber meshes only. Fabricated constructs remain viable for at least 3 weeks and maintain functionality as shown by inhibitor-sensitive transport activity, which suggests clearance capacity for both negatively and positively charged solutes.
Databáze: OpenAIRE
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