Studies on dynamic behavior of the biosensor based on immobilized glucoamylase-glucose oxidase membrane
| Autor: | Shinichiro Gondo, Michio Morishita, Chulsoon Kim, Satoshi Hirata |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Chromatography
Immobilized enzyme biology Chemistry Biomedical Engineering Biophysics Time constant Biosensing Techniques General Medicine Maltose Dead time Enzymes Immobilized Glucose Oxidase chemistry.chemical_compound Glucose Membrane Electrochemistry biology.protein Glucose oxidase Glucan 1 4-alpha-Glucosidase Biosensor Quantitative analysis (chemistry) Biotechnology |
| Zdroj: | Biosensors and Bioelectronics. 12:395-401 |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/s0956-5663(96)00082-6 |
| Popis: | Frequency response of the glucose/maltose biosensors based on immobilized glucoamylase and glucose oxidase membranes of different thickness were investigated with the sinusoidal signal generator of substrate concentration. The results were modeled by the serial combination of the dead time and the first order response blocks, and time constants and dead times were estimated. The estimated value of time constant ranged from 4.5 to 13.2 s and that of the dead time from 3.2 to 7.7 s with the tendency that both time constant and dead time increase as membrane thickness increases and number of enzymatic reaction steps increases. |
| Databáze: | OpenAIRE |
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