Evaluation of HER-2/neu Gene Status in Osteosarcoma by Fluorescence In Situ Hybridization and Multiplex and Monoplex Polymerase Chain Reactions
| Autor: | Sharon L. Hirschowitz, Joseph A. Holden, Dilip Gupta, Lester J. Layfield, Carl T. Wittwer, Carlynn Willmore-Payne, Holly Zhou |
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| Rok vydání: | 2006 |
| Předmět: |
Receptor
ErbB-2 Bone Neoplasms In situ hybridization Biology Polymerase Chain Reaction Pathology and Forensic Medicine chemistry.chemical_compound Gene duplication medicine Humans Multiplex Gene In Situ Hybridization Fluorescence Polymerase DNA Primers Osteosarcoma medicine.diagnostic_test Gene Amplification DNA Neoplasm General Medicine Genes erbB-2 medicine.disease Molecular biology Medical Laboratory Technology chemistry biology.protein Female DNA Probes DNA Fluorescence in situ hybridization |
| Zdroj: | Archives of Pathology & Laboratory Medicine. 130:691-698 |
| ISSN: | 1543-2165 0003-9985 |
| DOI: | 10.5858/2006-130-691-eongsi |
| Popis: | Context.—Previous reports suggest that the human epidermal growth factor 2 (HER-2/neu) receptor may be overexpressed in osteosarcoma. Objective.—To determine whether osteosarcomas have amplifications of the HER-2/neu gene. Design.—We studied a series of osteosarcomas by fluorescence in situ hybridization (FISH) and by 2 real-time polymerase chain reaction assays that measure the amount of HER-2/neu DNA relative to a control gene. The HER-2/ neu monoplex and multiplex assays were capable of identifying those cases of breast cancer that were known to overexpress HER-2/neu as assessed by FISH. We initially studied 21 cases of osteosarcoma by FISH analysis (using a technique that included a probe for chromosome 17), 11 of which had their HER-2/neu gene amplification status previously reported. Results.—None of these osteosarcoma cases showed HER-2/neu amplification by our FISH analysis and subsequent quantitative (multiplex) polymerase chain reaction. Apparent expression of HER-2/neu protein was observed in several of the cases but the immunoreactivity was localized to the cytoplasm and was not membranous in character. An additional 35 osteosarcoma specimens were subjected to monoplex polymerase chain reaction analysis, and amplifiable DNA was recovered from 19 specimens (54%). None of these samples had HER-2/neu amplification by monoplex PCR analysis and only one case had membranous immunoreactivity graded as 1+. Conclusion.—Although a small subset of osteosarcomas had weak noncircumferential membranous immunoreactivity for HER-2/neu protein, no osteosarcomas demonstrated positive (2+ or 3+) immunoreactivity for HER-2/ neu protein and none showed HER-2/neu gene amplification by either FISH or polymerase chain reaction. |
| Databáze: | OpenAIRE |
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