Structural determination of novel lacto-N-decaose and its monofucosylated analogue from human milk by electrospray tandem mass spectrometry and 1H NMR spectroscopy
| Autor: | V. E. Piskarev, Heide Kogelberg, Alexander M. Lawson, Wengang Chai, Yibing Zhang |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
chemistry.chemical_classification
Electrospray Spectrometry Mass Electrospray Ionization Chromatography Protein mass spectrometry Milk Human Molecular Structure Chemistry Electrospray ionization Molecular Sequence Data Biophysics Oligosaccharides Nuclear magnetic resonance spectroscopy Oligosaccharide Tandem mass spectrometry Mass spectrometry Biochemistry Methylation Carbohydrate Sequence Proton NMR Blood Group Antigens Humans Female Molecular Biology Nuclear Magnetic Resonance Biomolecular |
| Zdroj: | Archives of biochemistry and biophysics. 434(1) |
| ISSN: | 0003-9861 |
| Popis: | We have isolated and characterised two neutral oligosaccharides, one nonfucosylated and the other monofucosylated, from human milk that are based on the doubly branched lacto-N-decaose core. Their structures have been determined by a combined use of electrospray tandem mass spectrometry (ES-MS/MS) and NMR spectroscopy. The sequences of the three branches resulted from the double-branching, including the identity and location of the blood-group-related Lewis determinant and partial linkages, were elucidated by the unique method of high sensitivity negative-ion ES-MS/MS analysis. Their full structure assignment was completed by methylation analysis and 1H NMR. The monofucosylated lacto-N-decaose, Galbeta1-4(Fucalpha1-3)GlcNAcbeta1-6(Galbeta1-3GlcNAcbeta1-3)Galbeta1-4GlcNAcbeta1-6(Galbeta1-3GlcNAcbeta1-3)Galbeta1-4Glc is a novel sequence, whereas the nonfucosylated lacto-N-decaose, Galbeta1-4GlcNAcbeta1-6(Galbeta1-3GlcNAcbeta1-3)Galbeta1-4GlcNAcbeta1-6(Galbeta1-3GlcNAcbeta1-3)Galbeta1-4Glc, has not been isolated and identified as an individual oligosaccharide. |
| Databáze: | OpenAIRE |
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