The CKH2/PKL Chromatin Remodeling Factor Negatively Regulates Cytokinin Responses in Arabidopsis Calli
| Autor: | Kaori Furuta, Daisuke Shibata, Hiroo Fukuda, Tatsuo Kakimoto, Minoru Kubo, Kiyomi Sano, Yao-Guang Liu, Taku Demura |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Chloroplasts
Cytokinins Physiology Mutant Arabidopsis Chromatin Remodeling Factor Plant Science Genes Plant Hydroxamic Acids Plant Roots Histone Deacetylases Chromatin remodeling chemistry.chemical_compound Gene Expression Regulation Plant Two-Hybrid System Techniques Protein Interaction Mapping medicine Arabidopsis thaliana Photosynthesis Oligonucleotide Array Sequence Analysis biology Arabidopsis Proteins Genetic Complementation Test fungi DNA Helicases food and beverages Cell Biology General Medicine Chromatin Assembly and Disassembly Plants Genetically Modified biology.organism_classification Molecular biology Phenotype Trichostatin A chemistry Callus Mutation Cytokinin Transcription Factor TFIID Histone deacetylase Signal Transduction medicine.drug |
| Zdroj: | Plant and Cell Physiology. 52:618-628 |
| ISSN: | 1471-9053 0032-0781 |
| DOI: | 10.1093/pcp/pcr022 |
| Popis: | Cytokinins promote cell division and chloroplast development in tissue culture. We previously isolated two mutants of Arabidopsis thaliana, ckh1 (cytokinin-hypersensitive 1) and ckh2, which produce rapidly growing green calli in response to lower levels of cytokinins than those found in the wild type. Here we report that the product of the CKH2 gene is PICKLE, a protein resembling the CHD3 class of SWI/SNF chromatin remodeling factors. We also show that inhibition of histone deacetylase by trichostatin A (TSA) partially substituted for cytokinins, but not for auxin, in the promotion of callus growth, indicating that chromatin remodeling and histone deacetylation are intimately related to cytokinin-induced callus growth. A microarray experiment revealed that either the ckh1 mutation or the ckh2 mutation caused hypersensitivity to cytokinins in terms of gene expression, especially of photosynthesis-related genes. The ckh1 and ckh2 mutations up-regulated nuclear-encoded genes, but not plastid-encoded genes, whereas TSA deregulated both nuclear- and plastid-encoded genes. The ckh1 ckh2 double mutant showed synergistic phenotypes: the callus grew with a green color independently of exogenous cytokinins. A yeast two-hybrid experiment showed protein interaction between CKH1/EER4/AtTAF12b and CKH2/PKL. These results suggest that CKH1/EER4/AtTAF12b and CKH2/PKL may act together on cytokinin-regulated genes. |
| Databáze: | OpenAIRE |
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