Hypercholesterolemic patients have higher eryptosis and erythrocyte adhesion to human endothelium independently of statin therapy
| Autor: | María Amparo Blanch-Ruiz, Guadalupe Garcia-Llatas, M. A. Martínez-Cuesta, Antonio Cilla, Ángeles Álvarez, Reyes Barberá, Sergio Martínez-Hervás, José T. Real, Victor Collado-Diaz, Gabriel López-García |
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| Rok vydání: | 2021 |
| Předmět: |
Erythrocytes
Apolipoprotein B Endothelium Eryptosis Pharmacology medicine.disease_cause Microcirculation Flow cytometry chemistry.chemical_compound medicine Humans biology medicine.diagnostic_test business.industry Endothelial Cells General Medicine Glutathione Phosphatidylserine Adhesion Cross-Sectional Studies medicine.anatomical_structure chemistry biology.protein Calcium Hydroxymethylglutaryl-CoA Reductase Inhibitors business Oxidative stress |
| Zdroj: | International Journal of Clinical Practice. 75 |
| ISSN: | 1742-1241 1368-5031 |
| DOI: | 10.1111/ijcp.14771 |
| Popis: | BACKGROUND Phosphatidylserine (PS) externalization out of the membrane facilitates the eryptotic erythrocytes (EE) binding to endothelial cells (EC), potentially leading to atherosclerosis. Thus, the levels of eryptosis and interactions of EE-EC in hypercholesterolemic patients, either non-medicated or medicated, compared with healthy subjects were studied. METHODS A total of 56 subjects clustered into three groups: (control (n = 20), hypercholesterolemic non-treated (HCNT) (n = 15), and statin-treated (HCT) (n = 21)) were enrolled in this cross-sectional study. Biochemical parameters were determined with validated and standard methods. PS exposure was estimated from annexin-V-binding, cell volume from forward scatter (FSC), and GSH from CMFDA fluorescence by flow cytometry. The erythrocyte-EC adhesion assay was performed by using the parallel-plate flow chamber technique. RESULTS Higher PS externalization and adhesion of erythrocytes to EC (P |
| Databáze: | OpenAIRE |
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